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Literature summary for 1.6.1.3 extracted from
- Pyridine nucleotide transhydrogenase from spinach. I. Purification and properties (1960), J. Biol. Chem., 235, 2989-2996.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-adenylic acid | 43% inhibition, activity with NADPH and NAD+ | Spinacia oleracea |  |
| 3'-adenylic acid | 37% inhibition, activity with NADPH and NAD+ | Spinacia oleracea | |
| 5'-adenylic acid | 27% inhibition, activity with NADPH and NAD+ | Spinacia oleracea | |
| ADP | 48% inhibition, activity with NADPH and NAD+ | Spinacia oleracea | |
| Alpha-NAD+ | 33% inhibition, activity with NADPH and NAD+ | Spinacia oleracea | |
| ATP | 22% inhibition, activity with NADPH and NAD+ | Spinacia oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1 mM, slight stimulation | Spinacia oleracea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + NAD+ | Spinacia oleracea | the enzyme catalyses the NADPH-driven reduction of NAD+ | NADP+ + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, partially purified, stable for at least one month | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylpyridine-NADPH + NAD+ | stereospecificity with respect to NADPH is not known | Spinacia oleracea | acetylpyridine-NADP+ + NADH | - |
? | |
| deamino-NADPH + NAD+ | stereospecificity with respect to NADPH is not known | Spinacia oleracea | deamino-NADP+ + NADH | - |
? | |
| NADPH + acetylpyridine-NAD+ | stereospecificity with respect to NADPH is not known | Spinacia oleracea | NADP+ + acetylpyridine-NADH | - |
? | |
| NADPH + deamino-NAD+ | stereospecificity with respect to NADPH is not known | Spinacia oleracea | NADP+ + deamino-NADH | - |
? | |
| NADPH + NAD+ | the enzyme catalyses the NADPH-driven reduction of NAD+ | Spinacia oleracea | NADP+ + NADH | - |
? | |
| NADPH + NAD+ | stereospecificity with respect to NADPH is not known | Spinacia oleracea | NADP+ + NADH | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Spinacia oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
there is no loss in activity when the enzyme is left overnight at room temperature | Spinacia oleracea |
| 55 | - |
2.5 min, at least 90% of the activity is retained | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 9.4 | pH 7.6: about 40% of maximal activity, pH 9.4: about 70% of maximal activity | Spinacia oleracea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
no loss in activity after 15 min, 45% loss of activity after 4 hours | Spinacia oleracea |
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Release 2023.1 (January 2023)
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