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Literature summary for 1.6.1.2 extracted from

  • Ripoll, V.M.; Meadows, N.A.; Bangert, M.; Lee, A.W.; Kadioglu, A.; Cox, R.D.
    Nicotinamide nucleotide transhydrogenase (NNT) acts as a novel modulator of macrophage inflammatory responses (2012), FASEB J., 26, 3550-3562.
    View publication on PubMed
Search for more literature for 1.6.1.2

Cloned(Commentary)

Cloned (Comment) Organism
gene Nnt, overexpression of C-terminally V5-tagged full-length cDNA in the macrophage-like cell line RAW264.7, real-time PCR expression analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information enzyme overexpression reduces the production of inflammatory cytokines and the activation of mitogen-activated protein kinase signaling pathways in response to lipopolysaccharides. After 8 h, RAW264.7/NNT cells secrete significantly lower amounts of interleukin 6, TNF-alpha, and IL-1beta when compared to cells expressing empty vector. NNT overexpression impairs intracellular bacteria clearance. Wild-type C57BL/6J and C57BL/6J-NNT mice differed in inflammatory response within lung tissue Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
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 Mus musculus 5739
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Organism

Organism UniProt Comment Textmining
Mus musculus
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 gene Nnt
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Mus musculus C3H/HeJ
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 gene Nnt
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Mus musculus C57/BL6J
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 gene Nnt
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Source Tissue

Source Tissue Comment Organism Textmining
bone marrow high expression level Mus musculus
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brain
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 Mus musculus
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heart
-
 Mus musculus
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kidney
-
 Mus musculus
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liver high expression level Mus musculus
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lung high expression level Mus musculus
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macrophage bone marrow-derived, Nnt mRNA and protein are expressed in resting macrophages and down-regulated in response to inflammatory stimuli such as lipopolysaccharide. Nnt mRNA and protein expression are further repressed in fully activated macrophages Mus musculus
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macrophage bone marrow-derived, Nnt mRNA and protein are expressed in resting macrophages and down-regulated in response to inflammatory stimuli such as lipopolysaccharide. Nnt mRNA and protein expression are repressed in fully activated macrophages Mus musculus
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additional information quantitative PCR protein expression analysis Mus musculus
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pancreas
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 Mus musculus
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RAW-264.7 cell
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 Mus musculus
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skeletal muscle
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 Mus musculus
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spleen high expression level Mus musculus
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Synonyms

Synonyms Comment Organism
nicotinamide nucleotide transhydrogenase
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 Mus musculus
NNT
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 Mus musculus

General Information

General Information Comment Organism
malfunction C57BL/6J mice, which have a deletion in the Nnt gene, exhibit greater resistance to acute pulmonary infection with Streptococcus pneumoniae. Macrophages from these mice generate more reactive oxygen species and establish a stronger inflammatory response to this pathogen Mus musculus
physiological function nicotinamide nucleotide transhydrogenase, NNT, is a nuclear-encoded protein that functions as a redox-driven proton pump catalyzing the reversible reduction of NADP+ by NADH and conversion of NADH to NAD+. The enzyme acts as a modulator of macrophage inflammatory responses. The enzyme NNT is an efficient generator of NADPH required by glutathione reductase to reduce glutathione, a component necessary to decrease the buildup of reactive oxygen species Mus musculus