Literature summary for 1.6.1.2 extracted from

Kabus, A.; Georgi, T.; Wendisch, V.F.; Bott, M.
Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation (2007), Appl. Microbiol. Biotechnol., 75, 47-53.

Cloned(Commentary)

Cloned (Comment)	Organism
as Corynebacterium glutamicum does not possess membrane-integral nicotinamide nucleotide transhydrogenase, the Escherichia coli pntAB genes are expressed in the genetically defined Corynebacterium glutamicum lysine-producing strain DM1730, resulting in membrane-associated transhydrogenase activity of 0.7 U/mg proteine. Expression of the pntAB genes in Corynebacterium glutamicum improves L-lysine formation. In contrast, pntAB expression has a negative effect on growth and glutamate production of Corynebacterium glutamicum wild type	Escherichia coli

Cloned (Comment)

Organism

as Corynebacterium glutamicum does not possess membrane-integral nicotinamide nucleotide transhydrogenase, the Escherichia coli pntAB genes are expressed in the genetically defined Corynebacterium glutamicum lysine-producing strain DM1730, resulting in membrane-associated transhydrogenase activity of 0.7 U/mg proteine. Expression of the pntAB genes in Corynebacterium glutamicum improves L-lysine formation. In contrast, pntAB expression has a negative effect on growth and glutamate production of Corynebacterium glutamicum wild type

Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	bound	Escherichia coli	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NADP+ + NADH	Escherichia coli	the membrane-integral nicotinamide nucleotide transhydrogenase PntAB of Escherichia coli can use the electrochemical proton gradient across the cytoplasmic membrane to drive the reduction of NADP+ via the oxidation of NADH	NADPH + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADP+ + NADH	the membrane-integral nicotinamide nucleotide transhydrogenase PntAB of Escherichia coli can use the electrochemical proton gradient across the cytoplasmic membrane to drive the reduction of NADP+ via the oxidation of NADH	Escherichia coli	NADPH + NAD+	-	?
NADPH + 3-acetylpyridine-NAD+	-	Escherichia coli	3-acetylpyridine-NADH + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
PntAB	-	Escherichia coli