Cloned (Comment) | Organism |
---|---|
expression of domain I Y235N and Y235F mutants in Escherichia coli | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
Y235F | mutant domain I/wild-type domain III mixtures catalyse acetylpyridine adenine dinucleotide reduction with similar rates as wild-type domain I/wild-type domain III mixtures | Rhodospirillum rubrum |
Y235N | mutant domain I/wild-type domain III mixtures catalyse acetylpyridine adenine dinucleotide reduction with similar rates as wild-type domain I/wild-type domain III mixtures | Rhodospirillum rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + oxidized 3-acetylpyridine adenine dinucleotide | enzyme also catalyzes a rapid, so called cyclic reaction, i.e. the reduction of acetylpyridine adenine dinucleotide in the presence of either NADP+ or NADPH: the NADPH/NADP+ remain permanently bound to domain III and are alternately oxidized by acetylpyridine adenine dinucleotide and then reduced by NADH in domain I | Bos taurus | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
? | |
NADPH + oxidized 3-acetylpyridine adenine dinucleotide | enzyme also catalyzes a rapid, so called cyclic reaction, i.e. the reduction of acetylpyridine adenine dinucleotide in the presence of either NADP+ or NADPH: the NADPH/NADP+ remain permanently bound to domain III and are alternately oxidized by acetylpyridine adenine dinucleotide and then reduced by NADH in domain I | Rhodospirillum rubrum | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | domain I contains the binding site for NAD+ and NADH, domain III for NADP+ and NADPH | Rhodospirillum rubrum |
? | domain I exists as a seperate polypeptide that can be removed from everted membrane vesicle i.e. chromatophores | Rhodospirillum rubrum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bos taurus | |
NAD+ | - |
Rhodospirillum rubrum | |
NADH | - |
Bos taurus | |
NADH | - |
Rhodospirillum rubrum | |
NADP+ | - |
Bos taurus | |
NADP+ | - |
Rhodospirillum rubrum | |
NADPH | - |
Bos taurus | |
NADPH | - |
Rhodospirillum rubrum |