Cloned (Comment) | Organism |
---|---|
expression of domain I Y235N and Y235F mutants in Escherichia coli | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
Y235F | mutation resides in the soluble NAD(H)-binding peripheral membrane subunit, i.e. domain I, reconstitution of depleted membranes with mutant domain I gives 44% of activity that is obtained with wild-type domain I reconstituted membranes | Rhodospirillum rubrum |
Y235N | mutation resides in the soluble NAD(H)-binding peripheral membrane subunit, i.e. domain I, reconstitution of depleted membranes with mutant domain I gives 18% of activity that is obtained with wild-type domain I reconstituted membranes | Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADPH | Y235N and Y235F mutants of domain I | Rhodospirillum rubrum | |
0.03 | - |
NADPH | wild-type domain I | Rhodospirillum rubrum | |
0.06 | - |
oxidized 3-acetylpyridine adenine dinucleotide | wild-type domain I | Rhodospirillum rubrum | |
0.6 | - |
oxidized 3-acetylpyridine adenine dinucleotide | Y235F mutant of domain I | Rhodospirillum rubrum | |
0.8 | - |
oxidized 3-acetylpyridine adenine dinucleotide | Y235N mutant of domain I | Rhodospirillum rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + oxidized 3-acetylpyridine adenine dinucleotide | - |
Rhodospirillum rubrum | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhodospirillum rubrum | |
NADH | - |
Rhodospirillum rubrum | |
NADP+ | - |
Rhodospirillum rubrum | |
NADPH | - |
Rhodospirillum rubrum |