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Literature summary for 1.6.1.1 extracted from

  • French, C.E.; Boonstra, B.; Bufton, K.A.; Bruce, N.C.
    Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens (1997), J. Bacteriol., 179, 2761-2765.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000, SDS-PAGE Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography, native and recombinant enzyme Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, pH 7.0, 2 mM dithiothreitol, several weeks, no loss of activity, great loss of activity after several months Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADP+ + NADH
-
Pseudomonas fluorescens NADPH + NAD+
-
?

Subunits

Subunits Comment Organism
octamer x * 54000, SDS-PAGE Pseudomonas fluorescens