Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.99.B2 extracted from

  • Satomura, T.; Hara, Y.; Suye, S.; Sakuraba, H.; Ohshima, T.
    Gene expression and characterization of a third type of dye-linked L-proline dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix (2012), Biosci. Biotechnol. Biochem., 76, 589-593.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
oxidized 2,6-dichlorophenolindophenol pH 6.5, 50°C Aeropyrum pernix
0.28
-
L-proline pH 6.5, 50°C Aeropyrum pernix

Metals/Ions

Metals/Ions Comment Organism Structure
HgCl2 1 mM, 68% loss of activity Aeropyrum pernix
MgCl2 1 mM, 50% loss of activity Aeropyrum pernix
additional information no significant effect on activity with 1 mM SrCl2, CaCl2, CoCl2, MgCl2, CuCl2, ZnCl2 Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 47000, SDS-PAGE Aeropyrum pernix
88000
-
gel filtration Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aeropyrum pernix

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.57
-
pH 6.5, 50°C Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-hydroxyproline + oxidized 2,6-dichlorophenolindophenol + H2O reaction rate is 39% of that with L-proline Aeropyrum pernix ?
-
?
L-proline + oxidized 2,6-dichlorophenolindophenol + H2O the enzyme does not utilize ferricyanide or 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide as the electron acceptor, activity with phenazine methosulfate-p-iodonitrotetrazolium violet is 10% of that with 2,6-dichlorophenolindophenol Aeropyrum pernix (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
additional information no activity with: D-proline, glycine, L-glutamate, L-aspartate, L-arginine, L-lysine, L-serine, L-leucine, L-alanine, L-threonine, L-methionine,L-tryptophan, L-tyrosine Aeropyrum pernix ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 47000, SDS-PAGE Aeropyrum pernix

Synonyms

Synonyms Comment Organism
dye-linked L-proline dehydrogenase
-
Aeropyrum pernix
LPDH
-
Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Aeropyrum pernix
75
-
above Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
10 min, stable Aeropyrum pernix
100
-
10 min, 48% loss of activity Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Aeropyrum pernix

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 12 50°C, 30 min, stable Aeropyrum pernix

Cofactor

Cofactor Comment Organism Structure
FAD
-
Aeropyrum pernix