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Literature summary for 1.5.99.B2 extracted from

  • Kawakami, R.; Noguchi, C.; Higashi, M.; Sakuraba, H.; Ohshima, T.
    Comparative analysis of the catalytic components in the archaeal dye-linked L-proline dehydrogenase complexes (2013), Appl. Microbiol. Biotechnol., 97, 3419-3427.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells Pyrococcus furiosus
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells Pyrococcus horikoshii
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells Thermococcus kodakarensis
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells Thermococcus profundus

Inhibitors

Inhibitors Comment Organism Structure
L-pyroglutamate
-
Pyrococcus furiosus
L-pyroglutamate
-
Pyrococcus horikoshii
L-pyroglutamate
-
Thermococcus kodakarensis
L-pyroglutamate
-
Thermococcus profundus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.174
-
L-proline PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
0.334
-
L-proline PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
0.689
-
L-proline PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
1.46
-
L-proline TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
2.38
-
L-proline TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
2.46
-
L-proline TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus
2.5 5 L-proline PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
3.5
-
L-proline TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Pyrococcus horikoshii
-
-
-
Pyrococcus horikoshii OT-3
-
-
-
Thermococcus kodakarensis
-
-
-
Thermococcus kodakarensis KOD1 JCM 12380
-
-
-
Thermococcus profundus Q76M73
-
-
Thermococcus profundus DSM 9503 Q76M73
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelating column chromatography Pyrococcus furiosus
Ni-chelating column chromatography Pyrococcus horikoshii
Ni-chelating column chromatography Thermococcus kodakarensis
Ni-chelating column chromatography Thermococcus profundus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Pyrococcus furiosus DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Pyrococcus horikoshii DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Thermococcus kodakarensis DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Thermococcus profundus DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Thermococcus profundus DSM 9503 DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Pyrococcus horikoshii OT-3 DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Thermococcus kodakarensis KOD1 JCM 12380 DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
-
Pyrococcus furiosus DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
-
Pyrococcus horikoshii DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
-
Thermococcus kodakarensis DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
-
Thermococcus profundus DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
-
Thermococcus profundus DSM 9503 DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
-
Pyrococcus horikoshii OT-3 DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
-
Thermococcus kodakarensis KOD1 JCM 12380 DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
?
L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Pyrococcus furiosus DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
?
L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Pyrococcus horikoshii DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
?
L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Thermococcus kodakarensis DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
?
L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Thermococcus profundus DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
?
L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Thermococcus profundus DSM 9503 DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
?
L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Pyrococcus horikoshii OT-3 DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
?
L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Thermococcus kodakarensis KOD1 JCM 12380 DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
?
additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Pyrococcus furiosus ?
-
?
additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Pyrococcus horikoshii ?
-
?
additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Thermococcus kodakarensis ?
-
?
additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Thermococcus profundus ?
-
?
additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Thermococcus profundus DSM 9503 ?
-
?
additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Pyrococcus horikoshii OT-3 ?
-
?
additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Thermococcus kodakarensis KOD1 JCM 12380 ?
-
?

Subunits

Subunits Comment Organism
? x * 44000-45000, SDS-PAGE Pyrococcus furiosus
? x * 44000-45000, SDS-PAGE Pyrococcus horikoshii
? x * 44000-45000, SDS-PAGE Thermococcus kodakarensis
? x * 44000-45000, SDS-PAGE Thermococcus profundus

Synonyms

Synonyms Comment Organism
L-proline dehydrogenase
-
Pyrococcus furiosus
L-proline dehydrogenase
-
Pyrococcus horikoshii
L-proline dehydrogenase
-
Thermococcus kodakarensis
L-proline dehydrogenase
-
Thermococcus profundus
PDH
-
Pyrococcus furiosus
PDH
-
Pyrococcus horikoshii
PDH
-
Thermococcus kodakarensis
PDH
-
Thermococcus profundus
PF1246 alpha4beta4-type beta subunit of L-proline dehydrogenase Pyrococcus furiosus
PF1798 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Pyrococcus furiosus
PH1364 alpha4beta4-type beta subunit of L-proline dehydrogenase Pyrococcus horikoshii
PH1751 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Pyrococcus horikoshii
PRODH
-
Pyrococcus furiosus
PRODH
-
Pyrococcus horikoshii
PRODH
-
Thermococcus kodakarensis
PRODH
-
Thermococcus profundus
TK0117 alpha4beta4-type beta subunit of L-proline dehydrogenase Thermococcus kodakarensis
TK0122 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Thermococcus kodakarensis
TPpdhB alphabetagammadelta-type beta subunit of L-proline dehydrogenase Thermococcus profundus
TPpdhB2 alpha4beta4-type beta subunit of L-proline dehydrogenase Thermococcus profundus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
alphabetagammadelta-type beta subunit of L-proline dehydrogenase Pyrococcus furiosus
65
-
alphabetagammadelta-type beta subunit of L-proline dehydrogenase Pyrococcus horikoshii
65
-
alphabetagammadelta-type beta subunit of L-proline dehydrogenase Thermococcus kodakarensis
65
-
alphabetagammadelta-type beta subunit of L-proline dehydrogenase Thermococcus profundus
80
-
alpha4beta4-type beta subunit of L-proline dehydrogenase Pyrococcus furiosus
80
-
alpha4beta4-type beta subunit of L-proline dehydrogenase Pyrococcus horikoshii
80
-
alpha4beta4-type beta subunit of L-proline dehydrogenase Thermococcus kodakarensis
80
-
alpha4beta4-type beta subunit of L-proline dehydrogenase Thermococcus profundus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase nearly loses all of its activity under the same conditions Pyrococcus horikoshii
70
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase retains 100% activity under the same conditions Pyrococcus furiosus
70
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase shows 68% activity under the same conditions Thermococcus profundus
70
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase shows 76% activity under the same conditions Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.96
-
L-proline PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
3.49
-
L-proline PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
3.91
-
L-proline PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
6.22
-
L-proline TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus
11
-
L-proline PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
11.6
-
L-proline TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
27.6
-
L-proline TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
27.9
-
L-proline TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus

Cofactor

Cofactor Comment Organism Structure
FAD holoprotein contains one FAD per molecule Pyrococcus furiosus
FAD holoprotein contains one FAD per molecule Pyrococcus horikoshii
FAD holoprotein contains one FAD per molecule Thermococcus kodakarensis
FAD holoprotein contains one FAD per molecule Thermococcus profundus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
24
-
L-pyroglutamate PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
49.4
-
L-pyroglutamate TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
55.5
-
L-pyroglutamate PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
56.1
-
L-pyroglutamate TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus
68.3
-
L-pyroglutamate PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
122
-
L-pyroglutamate TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus
129
-
L-pyroglutamate PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
167
-
L-pyroglutamate TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis