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Literature summary for 1.5.99.4 extracted from
- Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans (1994), Mol. Microbiol., 13, 929-936.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Paenarthrobacter nicotinovorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Tungsten | inhibitory to enzyme activity, does not inhibit enzyme synthesis | Paenarthrobacter nicotinovorans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | present both as soluble and membrane-associated form | Paenarthrobacter nicotinovorans | 16020 | - |
| soluble | present both as soluble and membrane-associated form | Paenarthrobacter nicotinovorans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | subunit B carries an iron-sulfur cluster | Paenarthrobacter nicotinovorans | |
| Molybdenum | subunit C contains a molybdopterin-binding domain | Paenarthrobacter nicotinovorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14924 | - |
x * 30011, subunit A, x * 14924, subunit B, x * 87677, subunit C, calculated | Paenarthrobacter nicotinovorans |
| 30011 | - |
x * 30011, subunit A, x * 14924, subunit B, x * 87677, subunit C, calculated | Paenarthrobacter nicotinovorans |
| 87677 | - |
x * 30011, subunit A, x * 14924, subunit B, x * 87677, subunit C, calculated | Paenarthrobacter nicotinovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter nicotinovorans | Q93NH4 | encoded on megaplasmid pAO1 | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30011, subunit A, x * 14924, subunit B, x * 87677, subunit C, calculated | Paenarthrobacter nicotinovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDH | - |
Paenarthrobacter nicotinovorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | subunit A binds FAD | Paenarthrobacter nicotinovorans | |
| iron-sulfur centre | subunit B carries an iron-sulfur cluster | Paenarthrobacter nicotinovorans | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Paenarthrobacter nicotinovorans | expression of all subunits requires the presence of nicotine and molybdenum in the culture medium | up |
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