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Literature summary for 1.5.99.12 extracted from
- Kinetic and chemical analyses of the cytokinin dehydrogenase-catalysed reaction: correlations with the crystal structure (2006), Biochem. J., 398, 113-124.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kinetin + FAD | - |
Zea mays | 2-furaldehyde + adenine + FADH2 | - |
? |  |
| N-methyl-isopentenyladenine + FAD | - |
Zea mays | ? | - |
? | |
| N6-(2-isopentenyl)adenine + FAD | formation of a binary enzyme-product complex between the cytokinin imine and the reduced enzyme. The binary complex of the reduced enzyme and imine product intermediate decays relatively slowly to form an unbound product, cytokinin imine, which accumulates in the reaction mixture | Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
? | |
| p-topolin + FAD | - |
Zea mays | ? | - |
? | |
| trans-zeatin + FAD + H2O | - |
Zea mays | adenine + ? + FADH2 | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
N-methyl-isopentenyladenine | pH 6.5, 25°C | Zea mays | |
| 0.23 | - |
p-topolin | pH 6.5, 25°C | Zea mays | |
| 1.9 | - |
kinetin | pH 6.5, 25°C | Zea mays | |
| 40 | - |
N6-(2-isopentenyl)adenine | pH 6.5, 25°C | Zea mays | |
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