Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | relatively high concentrations of lyotropic salt is required for activity, 200fold activation | Methanopyrus kandleri |
Cloned (Comment) | Organism |
---|---|
gene mtd, DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli strain BL21(DE3) | Methanopyrus kandleri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
methylenetetrahydromethanopterin | - |
Methanosarcina barkeri | |
0.013 | - |
oxidized coenzyme F420 | - |
Archaeoglobus fulgidus | |
0.017 | - |
methylenetetrahydromethanopterin | - |
Archaeoglobus fulgidus | |
0.02 | - |
oxidized coenzyme F420 | native enzyme, pH 6.0, 65°C, N2-atmosphere | Methanopyrus kandleri | |
0.025 | - |
oxidized coenzyme F420 | - |
Methanosarcina barkeri | |
0.033 | - |
methylenetetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus | |
0.065 | - |
oxidized coenzyme F420 | - |
Methanothermobacter thermautotrophicus | |
0.08 | - |
methylenetetrahydromethanopterin | native enzyme, pH 6.0, 65°C, N2-atmosphere | Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29644 | - |
4 * 32000, approximately, SDS-PAGE, 4 * 29644, sequence calculation | Archaeoglobus fulgidus |
29644 | - |
6 * 36000, approximately, SDS-PAGE, 6 * 29644, sequence calculation | Methanothermobacter thermautotrophicus |
30305 | - |
x * 30305, sequence calculation | Methanocaldococcus jannaschii |
31000 | - |
8 * 31000, approximately, SDS-PAGE | Methanosarcina barkeri |
31383 | - |
8 * 36000, approximately, SDS-PAGE, 8 * 31383, sequence calculation | Methanopyrus kandleri |
32000 | - |
4 * 32000, approximately, SDS-PAGE, 4 * 29644, sequence calculation | Archaeoglobus fulgidus |
36000 | - |
6 * 36000, approximately, SDS-PAGE, 6 * 29644, sequence calculation | Methanothermobacter thermautotrophicus |
36000 | - |
8 * 36000, approximately, SDS-PAGE, 8 * 31383, sequence calculation | Methanopyrus kandleri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanosarcina barkeri | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
additional information | Methanothermobacter thermautotrophicus | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
additional information | Methanocaldococcus jannaschii | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
additional information | Archaeoglobus fulgidus | enzyme is involved in CO2 reduction to CH4 | ? | - |
? | |
additional information | Methanopyrus kandleri | enzyme is involved in CO2 reduction to CH4 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Methanopyrus kandleri | - |
hyperthermophilic archeon, methanogen, gene mtd | - |
Methanosarcina barkeri | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
O2 causes slow inactivation | Methanopyrus kandleri |
Purification (Comment) | Organism |
---|---|
native enzyme, multistep procedure involving ammonium sulfate fractionation and several chromatographic steps, recombinant from Escherichia coli strain BL21(DE3) | Methanopyrus kandleri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal growth at 37°C | Methanosarcina barkeri | - |
additional information | optimal growth at 65°C | Methanothermobacter thermautotrophicus | - |
additional information | optimal growth at 83°C | Archaeoglobus fulgidus | - |
additional information | optimal growth at 85°C | Methanocaldococcus jannaschii | - |
additional information | optimal growth on H2 and CO2 at 98°C | Methanopyrus kandleri | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Methanosarcina barkeri |
additional information | - |
- |
Methanothermobacter thermautotrophicus |
additional information | - |
- |
Archaeoglobus fulgidus |
additional information | - |
- |
Methanopyrus kandleri |
Storage Stability | Organism |
---|---|
-20°C, purified recombinant enzyme, N2-atmosphere, stable for at least 4 weeks | Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Methanosarcina barkeri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Methanothermobacter thermautotrophicus | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Methanocaldococcus jannaschii | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Archaeoglobus fulgidus | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is Si-face stereospecific with respect to C5 of F420 and Re-face stereospecific with respect to the methylene group of methylenetetrahydromethanopterin, reaction needs to be performed under strict anaerobic conditions | Methanopyrus kandleri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
additional information | enzyme is involved in CO2 reduction to CH4 | Methanosarcina barkeri | ? | - |
? | |
additional information | enzyme is involved in CO2 reduction to CH4 | Methanothermobacter thermautotrophicus | ? | - |
? | |
additional information | enzyme is involved in CO2 reduction to CH4 | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | enzyme is involved in CO2 reduction to CH4 | Archaeoglobus fulgidus | ? | - |
? | |
additional information | enzyme is involved in CO2 reduction to CH4 | Methanopyrus kandleri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30305, sequence calculation | Methanocaldococcus jannaschii |
hexamer | 6 * 36000, approximately, SDS-PAGE, 6 * 29644, sequence calculation | Methanothermobacter thermautotrophicus |
octamer | 8 * 31000, approximately, SDS-PAGE | Methanosarcina barkeri |
octamer | 8 * 36000, approximately, SDS-PAGE, 8 * 31383, sequence calculation | Methanopyrus kandleri |
tetramer | 4 * 32000, approximately, SDS-PAGE, 4 * 29644, sequence calculation | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
MTD | - |
Methanosarcina barkeri |
MTD | - |
Methanothermobacter thermautotrophicus |
MTD | - |
Methanocaldococcus jannaschii |
MTD | - |
Archaeoglobus fulgidus |
MTD | - |
Methanopyrus kandleri |
N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Methanosarcina barkeri |
N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Methanothermobacter thermautotrophicus |
N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Methanocaldococcus jannaschii |
N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Archaeoglobus fulgidus |
N5,N10-Methylenetetrahydromethanopterin dehydrogenase | - |
Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methanothermobacter thermautotrophicus |
60 | - |
above | Methanosarcina barkeri |
70 | - |
- |
Archaeoglobus fulgidus |
75 | - |
- |
Methanopyrus kandleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
30 min, stable up to over 90°C | Methanopyrus kandleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at, substrate methylenetetrahydromethanopterin, forward reaction | Methanopyrus kandleri |
8 | - |
assay at, substrate methenyltetrahydromethanopterin, reverse reaction | Methanopyrus kandleri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | dependent on | Methanosarcina barkeri | |
coenzyme F420 | dependent on | Methanothermobacter thermautotrophicus | |
coenzyme F420 | dependent on | Methanocaldococcus jannaschii | |
coenzyme F420 | dependent on | Archaeoglobus fulgidus | |
coenzyme F420 | dependent on | Methanopyrus kandleri | |
additional information | enzyme contains no prosthetic group | Methanopyrus kandleri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanopyrus kandleri | deduced from amino acid sequence | - |
4.2 |
Methanothermobacter thermautotrophicus | deduced from amino acid sequnce | - |
4.9 |
Archaeoglobus fulgidus | deduced from amino acid sequnce | - |
4.9 |
Methanocaldococcus jannaschii | deduced from amino acid sequnce | - |
6 |