Literature summary for 1.5.98.1 extracted from

Mukhopadhyay, B.; Purwantini, E.; Pihl, T.D.; Reeve, J.N.; Daniels, L.
Cloning, sequencing, and transcriptional analysis of the coenzyme F420-dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene from Methanobacterium thermoautotrophicum strain Marburg and functional expression in Escherichia coli (1995), J. Biol. Chem., 270, 2827-2832.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Methanothermobacter thermautotrophicus

Organism

Organism	UniProt	Comment	Textmining
Methanothermobacter thermautotrophicus	-	-	-
Methanothermobacter thermautotrophicus Marburg / DSM 2133	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420	-	Methanothermobacter thermautotrophicus	5,10-methenyltetrahydromethanopterin + reduced coenzyme F420	-	?
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420	-	Methanothermobacter thermautotrophicus Marburg / DSM 2133	5,10-methenyltetrahydromethanopterin + reduced coenzyme F420	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	55	recombinant enzyme	Methanothermobacter thermautotrophicus
55	65	native enzyme	Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	stable for more than 70 h, native and recombinant enzyme	Methanothermobacter thermautotrophicus
40	-	stable for more than 70 h, native and recombinant enzyme	Methanothermobacter thermautotrophicus
65	-	45% loss of activity in 1.5 h, 93% loss of activity in 24 h, recombinant enzyme. 35% loss of activity after 2 h, 96% loss of activity after 27 h	Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	native enzyme	Methanothermobacter thermautotrophicus
4.7	-	recombinant enzyme	Methanothermobacter thermautotrophicus

Cofactor

Cofactor	Comment	Organism	Structure
coenzyme F420	required	Methanothermobacter thermautotrophicus

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Release 2023.1 (January 2023)