KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
5,10-methylenetetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus | |
0.065 | - |
coenzyme F420 | - |
Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ | Methanothermobacter thermautotrophicus | |
K+ | monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ | Methanothermobacter thermautotrophicus | |
Li+ | monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ | Methanothermobacter thermautotrophicus | |
Na+ | monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ | Methanothermobacter thermautotrophicus | |
Na+ | 3 M NaCl stimulates 5fold | Methanothermobacter thermautotrophicus | |
NH4+ | monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
6 * 36000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus DELTAH | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | ternary complex mechanism | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
736 | - |
- |
Methanothermobacter thermautotrophicus |
Storage Stability | Organism |
---|---|
4°C, 0.5 mM 3-[(3-cholamidopropyl)dimethylammonio]1-propanesulfonate, stable for more than 6 months | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | r | Methanothermobacter thermautotrophicus | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | absolutely dependent on coenzyme F420 | Methanothermobacter thermautotrophicus | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | r | Methanothermobacter thermautotrophicus DELTAH | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | absolutely dependent on coenzyme F420 | Methanothermobacter thermautotrophicus DELTAH | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 36000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
oxidation of 5,10-methylenetetrahydromethanopterin | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | required | Methanothermobacter thermautotrophicus |