Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain JM109 as N-terminally His-tagged enzyme being equally distributed in the soluble fraction and the insoluble inclusion body fraction | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
N,N-dimethylglycine | pH 7.0, 30°C, recombinant holoenzyme, 2 Km-values | Rattus norvegicus | |
9.4 | - |
N,N-dimethylglycine | pH 7.0, 30°C, recombinant holoenzyme, 2 Km-values | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, native enzyme, SDS-PAGE, x * 93000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
93000 | - |
x * 90000, native enzyme, SDS-PAGE, x * 93000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + FADH2 | Rattus norvegicus | - |
5,10-methylene tetrahydrofolate + FAD | - |
? | |
N,N-dimethylglycine + H2O + FAD | Rattus norvegicus | - |
sarcosine + formaldehyde + FADH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats | - |
Purification (Comment) | Organism |
---|---|
native enzyme from liver mitochondria, recombinant His-tagged enzyme from Escherichia coli 45fold by nickel affinity chromatography | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
refolding by 80fold dilution after solubilization from inclusion bodies by urea treatment, 50-60% reactivation yield, overview | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.24 | - |
purified recombinant enzyme from soluble fraction | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + FADH2 | - |
Rattus norvegicus | 5,10-methylene tetrahydrofolate + FAD | - |
? | |
N,N-dimethylglycine + H2O + FAD | - |
Rattus norvegicus | sarcosine + formaldehyde + FADH2 | - |
? | |
N,N-dimethylglycine + H2O + oxidized 1-methoxy-5-methylphenazinium methylsulfate | - |
Rattus norvegicus | sarcosine + formaldehyde + reduced 1-methoxy-5-methylphenazinium methylsulfate | - |
? | |
N,N-dimethylglycine + H2O + oxidized 2,6-dichlorophenolindophenol | - |
Rattus norvegicus | sarcosine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, native enzyme, SDS-PAGE, x * 93000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
Me2GlyDH | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant and native enzyme | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant and native enzyme | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | absolutely required, covalently bound to the apoenzyme via a histidinyl(N3)-(8alpha)FAD linkage, cofactor-free apoenzyme shows 70% reduced activity compared to the holoenzyme | Rattus norvegicus | |
additional information | 1-methoxy-5-methylphenazinium methylsulfate can act as electron acceptor | Rattus norvegicus | |
additional information | 2,6-dichlorophenolindophenol can act as electron acceptor | Rattus norvegicus |