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Literature summary for 1.5.8.2 extracted from

  • Lu, X.; Nikolic, D.; Mitchell, D.J.; van Breemen, R.B.; Mersfelder, J.A.; Hille, R.; Silverman, R.B.
    A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase (2003), Bioorg. Med. Chem. Lett., 13, 4129-4132.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant C30A in Escherichia coli strain JM109 Methylophilus methylotrophus

Protein Variants

Protein Variants Comment Organism
C30A mutant shows highly increased activity to oxidate of the cofactor FMN to 6-hydroxyFMN compared to the wild-type enzyme Methylophilus methylotrophus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enzyme contains a [4Fe-4S] cluster Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trimethylamine + H2O + FMN Methylophilus methylotrophus
-
dimethylamine + formaldehyde + FMNH2
-
r

Organism

Organism UniProt Comment Textmining
Methylophilus methylotrophus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
trimethylamine + H2O + electron-transfer flavoprotein = dimethylamine + formaldehyde + reduced electron-transfer flavoprotein detailed reaction mechanism Methylophilus methylotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + O2 + H2O reaction mechanism, reoxidation of the cofactor, mutant C30A enzyme, low activity with wild-type enzyme and mutant W355L Methylophilus methylotrophus 6-hydroxy-FMN + H2O2 the hydroxy group oxygen is derived from molecular oxygen not from water r
trimethylamine + H2O + FMN
-
Methylophilus methylotrophus dimethylamine + formaldehyde + FMNH2
-
r
trimethylamine + H2O + FMN reaction mechanism Methylophilus methylotrophus dimethylamine + formaldehyde + FMNH2
-
r

Synonyms

Synonyms Comment Organism
More formerly EC 1.5.99.7 Methylophilus methylotrophus
TMADH
-
Methylophilus methylotrophus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
assay at Methylophilus methylotrophus

Cofactor

Cofactor Comment Organism Structure
FMN
-
Methylophilus methylotrophus