Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant C30A in Escherichia coli strain JM109 | Methylophilus methylotrophus |
Protein Variants | Comment | Organism |
---|---|---|
C30A | mutant shows highly increased activity to oxidate of the cofactor FMN to 6-hydroxyFMN compared to the wild-type enzyme | Methylophilus methylotrophus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enzyme contains a [4Fe-4S] cluster | Methylophilus methylotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + FMN | Methylophilus methylotrophus | - |
dimethylamine + formaldehyde + FMNH2 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trimethylamine + H2O + electron-transfer flavoprotein = dimethylamine + formaldehyde + reduced electron-transfer flavoprotein | detailed reaction mechanism | Methylophilus methylotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + O2 + H2O | reaction mechanism, reoxidation of the cofactor, mutant C30A enzyme, low activity with wild-type enzyme and mutant W355L | Methylophilus methylotrophus | 6-hydroxy-FMN + H2O2 | the hydroxy group oxygen is derived from molecular oxygen not from water | r | |
trimethylamine + H2O + FMN | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + FMNH2 | - |
r | |
trimethylamine + H2O + FMN | reaction mechanism | Methylophilus methylotrophus | dimethylamine + formaldehyde + FMNH2 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
More | formerly EC 1.5.99.7 | Methylophilus methylotrophus |
TMADH | - |
Methylophilus methylotrophus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Methylophilus methylotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Methylophilus methylotrophus |