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Literature summary for 1.5.8.2 extracted from

  • Colby, J.; Zatman, L.J.
    Purification and properties of the trimethylamine dehydrogenase of bacterium 4B6 (1974), Biochem. J., 143, 555-567.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.8.2

Inhibitors

Inhibitors Comment Organism Structure
1-phenylethylhydrazine potent inhibition at very low concentration methylotrophic bacterium
2-phenylcyclopropylamine 82% inhibition at 0.1 mM methylotrophic bacterium
2-phenylethylhydrazine potent inhibition at very low concentration methylotrophic bacterium
Ag+ strong inhibition at 1 mM methylotrophic bacterium
benzylhydrazine potent inhibition at very low concentration methylotrophic bacterium
Betaine hydrochloride 37% inhibition at 1 mM methylotrophic bacterium
choline chloride potent inhibition at 1 mM methylotrophic bacterium
Co2+ strong inhibition at 1 mM methylotrophic bacterium
Cu2+ strong inhibition at 1 mM methylotrophic bacterium
Harmaline 65% inhibition at 0.1 mM methylotrophic bacterium
hexamethylene bis-trimethylammonium chloride potent inhibition at 1 mM methylotrophic bacterium
Hg2+ strong inhibition at 1 mM methylotrophic bacterium
iodoacetamide inhibition at 1 mM methylotrophic bacterium
Iproniazid potent inhibition at very low concentration methylotrophic bacterium
isocarboxazid potent inhibition at very low concentration methylotrophic bacterium
isopropylhydrazine potent inhibition at very low concentration methylotrophic bacterium
N,N,N-trimethyl-N-phenylammonium chloride potent inhibition at 1 mM methylotrophic bacterium
N,N,N-trimethylhydrazonium iodide potent inhibition at 1 mM methylotrophic bacterium
N-benzyl-N-methylpropargylamine 27% inhibition at 0.1 mM methylotrophic bacterium
N-ethylmaleimide inhibition at 1 mM methylotrophic bacterium
Ni2+ strong inhibition at 1 mM methylotrophic bacterium
nialamid potent inhibition at very low concentration methylotrophic bacterium
p-chloromercuribenzoate inhibition at 1 mM methylotrophic bacterium
phenylhydrazine potent inhibition at very low concentration methylotrophic bacterium
Tetramethylammonium chloride potent inhibition at 1 mM methylotrophic bacterium
trimethylamine inhibitory at higher concentrations methylotrophic bacterium
trimethylsulfomium chloride potent inhibition at 1 mM methylotrophic bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 trimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
0.002
-
 ethyldimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
0.002
-
 2-chloroethyldimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
0.006
-
 2-hydroxyethyldimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
0.008
-
 diethylmethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
0.067
-
 2-aminoethyldimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
0.13
-
 triethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
1.25
-
 phenazine methosulfate substrate: trimethylamine methylotrophic bacterium
1.7
-
 Diethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
161000
-
 gel filtration methylotrophic bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trimethylamine + H2O + electron acceptor methylotrophic bacterium electron acceptors: phenazine methosulfate, brilliant cresyl blue, methylene blue dimethylamine + formaldehyde + reduced electron acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
methylotrophic bacterium
-
-
-
methylotrophic bacterium 4B6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps methylotrophic bacterium

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 methylotrophic bacterium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
-
 methylotrophic bacterium

Storage Stability

Storage Stability Organism
-20°C, 20 mM sodium phosphate, pH 7.5, 0.4 mg protein/ml, stable for 3 months methylotrophic bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminoethyldimethylamine + H2O + phenazine methosulfate 10% of the activity compared to trimethylamine methylotrophic bacterium ? + reduced phenazine methosulfate
-
 ?
2-aminoethyldimethylamine + H2O + phenazine methosulfate 10% of the activity compared to trimethylamine methylotrophic bacterium 4B6 ? + reduced phenazine methosulfate
-
 ?
2-chloroethyldimethylamine + H2O + phenazine methosulfate 40% of the activity compared to trimethylamine methylotrophic bacterium ? + reduced phenazine methosulfate
-
 ?
2-hydroxyethyldimethylamine + H2O + phenazine methosulfate 70% of the activity compared to trimethylamine methylotrophic bacterium ? + reduced phenazine methosulfate
-
 ?
diethylamine + H2O + phenazine methosulfate 47% of the activity compared to trimethylamine methylotrophic bacterium ethylamine + acetaldehyde + reduced phenazine methosulfate
-
 ?
diethylamine + H2O + phenazine methosulfate 47% of the activity compared to trimethylamine methylotrophic bacterium 4B6 ethylamine + acetaldehyde + reduced phenazine methosulfate
-
 ?
diethylmethylamine + H2O + phenazine methosulfate 11% of the activity compared to trimethylamine methylotrophic bacterium diethylamine + ethylmethylamine + formaldehyde + acetaldehyde + reduced phenazine methosulfate
-
 ?
diethylmethylamine + H2O + phenazine methosulfate 11% of the activity compared to trimethylamine methylotrophic bacterium 4B6 diethylamine + ethylmethylamine + formaldehyde + acetaldehyde + reduced phenazine methosulfate
-
 ?
ethyldimethylamine + H2O + phenazine methosulfate 16% of the activity compared to trimethylamine methylotrophic bacterium ethylmethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?
ethyldimethylamine + H2O + phenazine methosulfate 16% of the activity compared to trimethylamine methylotrophic bacterium 4B6 ethylmethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?
triethylamine + H2O + phenazine methosulfate 3% of the activity compared to trimethylamine methylotrophic bacterium diethylamine + acetaldehyde + reduced phenazine methosulfate
-
 ?
trimethylamine + H2O + electron acceptor electron acceptors: phenazine methosulfate, brilliant cresyl blue, methylene blue methylotrophic bacterium dimethylamine + formaldehyde + reduced electron acceptor
-
 ?
trimethylamine + H2O + phenazine methosulfate
-
 methylotrophic bacterium dimethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?
trimethylamine + H2O + phenazine methosulfate
-
 methylotrophic bacterium 4B6 dimethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 loss of 50% of activity in 20 min methylotrophic bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 methylotrophic bacterium