Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetaldehyde | noncompetitive inhibition with respect to diethylamine, uncompetitive inhibition with respect to phenazine methosulfate | Methylophilus methylotrophus | |
Diethylamine | noncompetitive product inhibition with respect to phenazine methosulfate | Methylophilus methylotrophus | |
ethylamine | noncompetitive product inhibition with respect to phenazine methosulfate | Methylophilus methylotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
146800 | - |
sedimentation equilibrium ultracentrifugation | Methylophilus methylotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + electron acceptor | Methylophilus methylotrophus | ping-ping reaction mechanism | dimethylamine + formaldehyde + reduced electron acceptor | - |
? | |
trimethylamine + H2O + electron acceptor | Methylophilus methylotrophus W3A1 | ping-ping reaction mechanism | dimethylamine + formaldehyde + reduced electron acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Methylophilus methylotrophus W3A1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Methylophilus methylotrophus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Methylophilus methylotrophus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.07 | - |
- |
Methylophilus methylotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethylamine + H2O + phenazine methosulfate | - |
Methylophilus methylotrophus | ethylamine + acetaldehyde + reduced phenazine methosulfate | - |
? | |
diethylamine + H2O + phenazine methosulfate | - |
Methylophilus methylotrophus W3A1 | ethylamine + acetaldehyde + reduced phenazine methosulfate | - |
? | |
trimethylamine + H2O + 2,6-dichlorophenolindophenol | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
trimethylamine + H2O + 2,6-dichlorophenolindophenol | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
trimethylamine + H2O + electron acceptor | ping-ping reaction mechanism | Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced electron acceptor | - |
? | |
trimethylamine + H2O + electron acceptor | ping-ping reaction mechanism | Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced electron acceptor | - |
? | |
trimethylamine + H2O + phenazine methosulfate | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced phenazine methosulfate | - |
? | |
trimethylamine + H2O + phenazine methosulfate | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced phenazine methosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | non-identical subunits of 70000-80000, SDS-PAGE | Methylophilus methylotrophus |