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Literature summary for 1.5.8.2 extracted from

  • Steenkamp, D.J.; Mallinson, J.
    Trimethylamine dehydrogenase from a methylotrophic bacterium. I. Isolation and steady-state kinetics (1976), Biochim. Biophys. Acta, 429, 7005-719.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde noncompetitive inhibition with respect to diethylamine, uncompetitive inhibition with respect to phenazine methosulfate Methylophilus methylotrophus
Diethylamine noncompetitive product inhibition with respect to phenazine methosulfate Methylophilus methylotrophus
ethylamine noncompetitive product inhibition with respect to phenazine methosulfate Methylophilus methylotrophus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
146800
-
sedimentation equilibrium ultracentrifugation Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trimethylamine + H2O + electron acceptor Methylophilus methylotrophus ping-ping reaction mechanism dimethylamine + formaldehyde + reduced electron acceptor
-
?
trimethylamine + H2O + electron acceptor Methylophilus methylotrophus W3A1 ping-ping reaction mechanism dimethylamine + formaldehyde + reduced electron acceptor
-
?

Organism

Organism UniProt Comment Textmining
Methylophilus methylotrophus
-
-
-
Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Methylophilus methylotrophus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Methylophilus methylotrophus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.07
-
-
Methylophilus methylotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethylamine + H2O + phenazine methosulfate
-
Methylophilus methylotrophus ethylamine + acetaldehyde + reduced phenazine methosulfate
-
?
diethylamine + H2O + phenazine methosulfate
-
Methylophilus methylotrophus W3A1 ethylamine + acetaldehyde + reduced phenazine methosulfate
-
?
trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
Methylophilus methylotrophus dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
trimethylamine + H2O + electron acceptor ping-ping reaction mechanism Methylophilus methylotrophus dimethylamine + formaldehyde + reduced electron acceptor
-
?
trimethylamine + H2O + electron acceptor ping-ping reaction mechanism Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced electron acceptor
-
?
trimethylamine + H2O + phenazine methosulfate
-
Methylophilus methylotrophus dimethylamine + formaldehyde + reduced phenazine methosulfate
-
?
trimethylamine + H2O + phenazine methosulfate
-
Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced phenazine methosulfate
-
?

Subunits

Subunits Comment Organism
dimer non-identical subunits of 70000-80000, SDS-PAGE Methylophilus methylotrophus