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Literature summary for 1.5.8.2 extracted from

  • Mitchell, D.J.; Nikolic, D.; Jang, M.H.; van Breemen, R.B.; Hille, R.; Silverman, R.B.
    Inactivation of C30A trimethylamine dehydrogenase by N-cyclopropyl-alpha-methylbenzylamine, 1-phenylcyclopropylamine, and phenylhydrazine (2001), Biochemistry, 40, 8523-8530.
    View publication on PubMed
Search for more literature for 1.5.8.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

Protein Variants Comment Organism
C30A binds FMN non-covalently Methylophilus methylotrophus

Inhibitors

Inhibitors Comment Organism Structure
1-phenylcyclopropylamine irreversible inactivation of both wild-type and mutant C30A Methylophilus methylotrophus
N-cyclopropyl-alpha-methylbenzylamine irreversible inactivation of both wild-type and mutant C30A, more potent than 1-phenylcyclopropylamine Methylophilus methylotrophus
phenylhydrazine irreversible inactivation of both wild-type and mutant C30A, 10 times more potent than N-cyclopropyl-alpha-methylbenzylamine Methylophilus methylotrophus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe
-
 Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus
-
 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?
trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus W3A1
-
 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Methylophilus methylotrophus
-
-
-
Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Methylophilus methylotrophus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Methylophilus methylotrophus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trimethylamine + H2O + electron transferring flavoprotein
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?
trimethylamine + H2O + electron transferring flavoprotein
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Methylophilus methylotrophus