Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methylophilus methylotrophus |
Protein Variants | Comment | Organism |
---|---|---|
C-terminal truncations | C-terminal truncations of 5, 10 or 17 residues do not affect dimer stability, but compromise the ability of the enzyme to become covalently coupled with FMN in the active site | Methylophilus methylotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
trimethylamine | mutant DELTA725-729, electron acceptor: phenazine methosulfate | Methylophilus methylotrophus | |
0.0047 | - |
trimethylamine | mutant DELTA720-729, electron acceptor: phenazine methosulfate | Methylophilus methylotrophus | |
0.0096 | - |
trimethylamine | recombinant wild-type form, electron acceptor: phenazine methosulfate | Methylophilus methylotrophus | |
0.0137 | - |
trimethylamine | native wild-type form, electron acceptor: phenazine methosulfate | Methylophilus methylotrophus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 4Fe-4S-center | Methylophilus methylotrophus | |
Fe | 4.5 mol iron per mol enzyme | Methylophilus methylotrophus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Methylophilus methylotrophus W3A1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Methylophilus methylotrophus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Methylophilus methylotrophus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + 2,6-dichlorophenolindophenol | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
trimethylamine + H2O + 2,6-dichlorophenolindophenol | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
trimethylamine + H2O + phenazine methosulfate | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced phenazine methosulfate | - |
? | |
trimethylamine + H2O + phenazine methosulfate | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced phenazine methosulfate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
trimethylamine | mutant delta725-729, electron acceptor: phenazine methosulfate | Methylophilus methylotrophus | |
6.4 | - |
trimethylamine | mutant delta720-729, electron acceptor: phenazine methosulfate | Methylophilus methylotrophus | |
10.2 | - |
trimethylamine | recombinant wild-type form, electron acceptor: phenazine methosulfate | Methylophilus methylotrophus | |
15.6 | - |
trimethylamine | native wild-type form, electron acceptor: phenazine methosulfate | Methylophilus methylotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Methylophilus methylotrophus | |
FMN | - |
Methylophilus methylotrophus |