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Literature summary for 1.5.8.2 extracted from

  • Huang, L.; Scrutton, N.S.; Hille, R.
    Reaction of the C30A mutant of trimethylamine dehydrogenase with diethylmethylamine (1996), J. Biol. Chem., 271, 13401-13406.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

Protein Variants Comment Organism
C30A removal of the 6-S-cysteinyl-FMN bond, diminishes limiting rate for the first of three observed kinetic phases Methylophilus methylotrophus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 4Fe-4S-center Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diethylmethylamine + H2O + electron acceptor Methylophilus methylotrophus slow substrate, triphasic reaction kinetic diethylamine + formaldehyde + reduced electron acceptor
-
?
diethylmethylamine + H2O + electron acceptor Methylophilus methylotrophus W3A1 slow substrate, triphasic reaction kinetic diethylamine + formaldehyde + reduced electron acceptor
-
?

Organism

Organism UniProt Comment Textmining
Methylophilus methylotrophus
-
-
-
Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Methylophilus methylotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethylmethylamine + H2O + electron acceptor slow substrate, triphasic reaction kinetic Methylophilus methylotrophus diethylamine + formaldehyde + reduced electron acceptor
-
?
diethylmethylamine + H2O + electron acceptor slow substrate, triphasic reaction kinetic Methylophilus methylotrophus W3A1 diethylamine + formaldehyde + reduced electron acceptor
-
?

Cofactor

Cofactor Comment Organism Structure
FMN covalently bound to Cys30 Methylophilus methylotrophus