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Literature summary for 1.5.5.2 extracted from

  • Zhang, M.; White, T.A.; Schuermann, J.P.; Baban, B.A.; Becker, D.F.; Tanner, J.J.
    Structures of the Escherichia coli PutA proline dehydrogenase domain in complex with competitive inhibitors (2004), Biochemistry, 43, 12539-12548.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vaor diffusion method, structures of the PutA PRODH domain complexed with competitive inhibitors, acetate, L-lactate and L-tetrahydro-2-furoic acid Escherichia coli

Protein Variants

Protein Variants Comment Organism
L432P mutant form of PutA86-669, 5fold decrease in turnover-number and a severe loss in thermostability Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
acetate competitive Escherichia coli
L-lactate competitive Escherichia coli
L-tetrahydro-2-furoic acid competitive Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
L-proline 25°C, wild-type PutA86-669 Escherichia coli
0.13
-
L-proline 25°C, L432P mutant form of PutA86-669 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
gel electrophoresis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-proline + acceptor + H2O Escherichia coli the enzyme catalyzes the first step of proline catabolism (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P09546 PutA86-669
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + acceptor + H2O the enzyme catalyzes the first step of proline catabolism Escherichia coli (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
PRODH
-
Escherichia coli
PutA proline dehydrogenase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
50% loss of activity of wild-type PutA86-669 after 1 h, 50% loss of of L432P mutant form of PutA86-669 after 8 min Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
L-proline 25°C, L432P mutant form of PutA86-669 Escherichia coli
17
-
L-proline 25°C, wild-type PutA86-669 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
L-tetrahydro-2-furoic acid
-
Escherichia coli
1
-
L-lactate
-
Escherichia coli
30
-
acetate
-
Escherichia coli