Any feedback?
Literature summary for 1.5.5.1 extracted from
- Expression of human electron transfer flavoprotein-ubiquinone oxidoreductase from baculovirus vector: kinetic and spectral characterization of the human protein (2002), Biochem. J., 364, 659-667.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Sf9 insect cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00013 | - |
electron-transferring flavoprotein | - |
Homo sapiens |  |
| 0.0049 | - |
ubiquinone-2 | - |
Homo sapiens | |
| 0.0081 | - |
ubiquinone-1 | - |
Homo sapiens | |
| 0.0084 | - |
decylubiquinone | - |
Homo sapiens | |
| 0.0148 | - |
ubiquinone-4 | - |
Homo sapiens | |
| 0.0154 | - |
semiquinone electron-transferring flavoprotein | - |
Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
x * 64000, assumed to be a monomer in the mitochondrial membrane, immunoblot | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, DEAE-Sepharose, HA-Ultrogel, Mono Q | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.6 | - |
recombinant enzyme, ubiquinone-1 reduction | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced electron-transferring flavoprotein + 2,3-dimethoxy-5-methyl-6-(3-methylbut-2-en)-1,4-benzoquinone | - |
Homo sapiens | electron-transferring flavoprotein + 2,3-dimethoxy-5-methyl-6-(3-methylbut-2-en)-1,4-benzoquinol | - |
? | |
| reduced electron-transferring flavoprotein + decylubiquinone | - |
Homo sapiens | electron-transferring flavoprotein + decylubiquinol | - |
? | |
| reduced electron-transferring flavoprotein + ubiquinone-2 | - |
Homo sapiens | electron-transferring flavoprotein + ubiquinol-2 | - |
? | |
| reduced electron-transferring flavoprotein + ubiquinone-4 | - |
Homo sapiens | electron-transferring flavoprotein + ubiquinol-4 | - |
? | |
| semiquinone electron transferring flavoprotein + ubiquinone Q1 | - |
Homo sapiens | electron-transferring flavoprotein + hydroquinone electron transferring flavoprotein + ubiquinol Q1 | - |
r | |
| semiquinone electron-transferring flavoprotein + ubiquinone Q1 | - |
Homo sapiens | electron-transferring flavoprotein + hydroquinone electron-transferring flavoprotein + ubiquinol Q1 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, assumed to be a monomer in the mitochondrial membrane, immunoblot | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.4 | - |
electron-transferring flavoprotein | - |
Homo sapiens | |
| 33.5 | - |
ubiquinone-1 | - |
Homo sapiens | |
| 35.5 | - |
ubiquinone-4 | - |
Homo sapiens | |
| 74.3 | - |
decylubiquinone | - |
Homo sapiens | |
| 78.8 | - |
ubiqinone-2 | - |
Homo sapiens | |
| 81.4 | - |
semiquinone electron-transferring flavoprotein | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
