Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 | Paenarthrobacter nicotinovorans |
Protein Variants | Comment | Organism |
---|---|---|
C136S | 5% of wild-type activity, slight activation with glycerol-3-phosphate and FAD | Paenarthrobacter nicotinovorans |
C136S/C260S | no activity, no activation with glycerol-3-phosphate and FAD | Paenarthrobacter nicotinovorans |
C260S | 15% of wild-type activity, no activation with glycerol-3-phosphate and FAD | Paenarthrobacter nicotinovorans |
C433S | 40% of wild-type activity, 50% activation with glycerol-3-phosphate and FAD | Paenarthrobacter nicotinovorans |
C433S | deletions F448 and R449, no activity, no activation with glycerol-3-phosphate and FAD | Paenarthrobacter nicotinovorans |
C59S | 40% of wild-type activity, no activation with glycerol-3-phosphate and FAD | Paenarthrobacter nicotinovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dithionitrobenzoic acid | inhibits holoenzyme formation from apoenzyme and FAD, can be prevented by addition of 2-mercaptoethanol | Paenarthrobacter nicotinovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavinylation can be performed with purified apoenzyme post-translationally with chaperonins ATP, GroEL and GroES | Paenarthrobacter nicotinovorans |