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Literature summary for 1.5.3.17 extracted from

  • Tormos, J.; Henderson Pozzi, M.; Fitzpatrick, P.
    Mechanistic studies of the role of a conserved histidine in a mammalian polyamine oxidase (2012), Arch. Biochem. Biophys., 528, 45-49.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.3.17

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes Mus musculus

Protein Variants

Protein Variants Comment Organism
H64A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Mus musculus
H64N site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Mus musculus
H64Q site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Mus musculus
0.027
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant wild-type enzyme Mus musculus
0.04
-
 spermine pH 10.0, 30°C, recombinant mutant H64N Mus musculus
0.05
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64A Mus musculus
0.053
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64Q Mus musculus
0.07
-
 O2 pH 10.0, 30°C, recombinant wild-type enzyme, with spermine Mus musculus
0.13
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant mutant H64Q Mus musculus
0.15
-
 O2 pH 8.6, 30°C, recombinant mutants H64A and H64Q, with N,N'-dibenzyl-1,4-diaminobutane Mus musculus
0.16
-
 spermine pH 10.0, 30°C, recombinant wild-type enzyme Mus musculus
0.16
-
 O2 pH 8.6, 30°C, recombinant wild-type enzyme, with N,N'-dibenzyl-1,4-diaminobutane Mus musculus
0.2
-
 O2 pH 8.6, 30°C, recombinant mutant H64N, with N,N'-dibenzyl-1,4-diaminobutane Mus musculus
0.21
-
 N1-acetylspermine pH 10.0, 30°C, recombinant wild-type enzyme Mus musculus
0.21
-
 spermine pH 10.0, 30°C, recombinant mutants H64A and H64Q Mus musculus
0.24
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64N Mus musculus
0.27
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant mutant H64A Mus musculus
0.3
-
 O2 pH 10.0, 30°C, recombinant mutants H64A and H64N, with spermine Mus musculus
0.37
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant mutant H64N Mus musculus
0.5
-
 O2 pH 10.0, 30°C, recombinant mutant H64Q, with spermine Mus musculus
39
-
 O2 pH 10.0, 30°C, recombinant wild-type enzyme and recombinant mutants H64A and H64N, with N1-acetylspermine Mus musculus
53
-
 O2 pH 10.0, 30°C, recombinant mutant H64Q, with N1-acetylspermine Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Mus musculus 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N'-dibenzyl-1,4-diaminobutane + O2 + H2O Mus musculus slow substrate ? + H2O2
-
 ?
N1-acetylspermine + O2 + H2O Mus musculus
-
 spermidine + 3-acetamidopropanal + H2O2
-
 ?
spermine + O2 + H2O Mus musculus slow substrate spermidine + 3-aminopropanal + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N'-dibenzyl-1,4-diaminobutane + O2 + H2O slow substrate Mus musculus ? + H2O2
-
 ?
N1-acetylspermine + O2 + H2O
-
 Mus musculus spermidine + 3-acetamidopropanal + H2O2
-
 ?
spermine + O2 + H2O slow substrate Mus musculus spermidine + 3-aminopropanal + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
PAO
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 spermine pH 10.0, 30°C, recombinant mutant H64N Mus musculus
0.16
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant mutant H64A Mus musculus
0.2
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant mutant H64Q Mus musculus
0.22
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant mutant H64N Mus musculus
0.3
-
 spermine pH 10.0, 30°C, recombinant mutant H64A Mus musculus
0.34
-
 spermine pH 10.0, 30°C, recombinant mutant H64Q Mus musculus
0.9
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant wild-type enzyme Mus musculus
3.1
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64A Mus musculus
4.1
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64N Mus musculus
4.5
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64Q Mus musculus
4.7
-
 spermine pH 10.0, 30°C, recombinant wild-type enzyme Mus musculus
49
-
 N1-acetylspermine pH 10.0, 30°C, recombinant wild-type enzyme Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6 10 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Mus musculus

General Information

General Information Comment Organism
additional information His64 hydrogen bonds to the reactive nitrogen in the polyamine substrate Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.08
-
 O2 pH 10.0, 30°C, recombinant mutant H64A, with N1-acetylspermine Mus musculus
0.09
-
 O2 pH 10.0, 30°C, recombinant mutant H64Q, with N1-acetylspermine Mus musculus
0.11
-
 O2 pH 10.0, 30°C, recombinant mutant H64N, with N1-acetylspermine Mus musculus
0.4
-
 O2 pH 10.0, 30°C, recombinant mutant H64N, with spermine Mus musculus
0.6
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant mutants H64A and H64N Mus musculus
0.7
-
 O2 pH 10.0, 30°C, recombinant mutant H64Q, with spermine Mus musculus
1
-
 O2 pH 10.0, 30°C, recombinant mutants H64A and H64N, with spermine Mus musculus
1
-
 O2 pH 8.6, 30°C, recombinant mutant H64A, with N,N'-dibenzyl-1,4-diaminobutane Mus musculus
1.1
-
 O2 pH 8.6, 30°C, recombinant mutant H64N, with N,N'-dibenzyl-1,4-diaminobutane Mus musculus
1.3
-
 O2 pH 10.0, 30°C, recombinant wild-type enzyme, with N1-acetylspermine Mus musculus
1.4
-
 spermine pH 10.0, 30°C, recombinant mutant H64A Mus musculus
1.4
-
 O2 pH 8.6, 30°C, recombinant mutant H64Q, with N,N'-dibenzyl-1,4-diaminobutane Mus musculus
1.6
-
 spermine pH 10.0, 30°C, recombinant mutant H64N Mus musculus
1.6
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant mutant H64Q Mus musculus
3
-
 spermine pH 10.0, 30°C, recombinant mutant H64Q Mus musculus
5.5
-
 O2 pH 8.6, 30°C, recombinant wild-type enzyme, with N,N'-dibenzyl-1,4-diaminobutane Mus musculus
17
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64N Mus musculus
29
-
 spermine pH 10.0, 30°C, recombinant wild-type enzyme Mus musculus
33
-
 N,N'-dibenzyl-1,4-diaminobutane pH 8.6, 30°C, recombinant wild-type enzyme Mus musculus
62
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64A Mus musculus
66
-
 O2 pH 10.0, 30°C, recombinant wild-type enzyme, with spermine Mus musculus
84
-
 N1-acetylspermine pH 10.0, 30°C, recombinant mutant H64Q Mus musculus
230
-
 N1-acetylspermine pH 10.0, 30°C, recombinant wild-type enzyme Mus musculus