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Literature summary for 1.5.3.17 extracted from

  • Müller, S.; Walter, R.D.
    Purification and characterization of polyamine oxidase from Ascaris suum (1992), Biochem. J., 1283, 75-80.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
dithioerythritol up to 0.010 mM increase the enzyme activity, higher concentrations inhibit Ascaris suum
dithiothreitol up to 0.010 mM increase the enzyme activity, higher concentrations inhibit Ascaris suum
Iproniazid 0.01 mM, 87% inhibition Ascaris suum
isoniazid 0.01 mM, 81% inhibition Ascaris suum
MDL 27391
-
Ascaris suum
MDL 27695
-
Ascaris suum
additional information the polyamine oxidase inhibitor MDL 72527 has no effect on the parasite polyamine oxidase activity Ascaris suum
NEM 0.01 mM, 85% inhibition Ascaris suum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
spermine pH 8.5, 37°C Ascaris suum
0.66
-
spermidine pH 8.5, 37°C Ascaris suum
1.6
-
benzylamidine pH 8.5, 37°C Ascaris suum
2
-
norspermine pH 8.5, 37°C Ascaris suum
2.5
-
tryptamine pH 8.5, 37°C Ascaris suum
5
-
norspermidine pH 8.5, 37°C Ascaris suum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information copper and iron are not cofactors Ascaris suum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
x * 66000, SDS-PAGE Ascaris suum

Organism

Organism UniProt Comment Textmining
Ascaris suum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ascaris suum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
-
Ascaris suum

Storage Stability

Storage Stability Organism
-20°C, stable for 2 days (minimal loss of activity), complete loss of enzyme activity after 14 days Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylamidine + O2 + H2O
-
Ascaris suum ?
-
?
additional information N-acetylated polyamines and diamines, e.g. N1-acetylspermidine, N8-acetylspermidine, N-acetylspermine, N1,N12-diacetylspermine, putrescine, cadaverine and histamine, are not accepted as substrates. Bis(benzyl)polyamines, such as MDL 27695 and MDL 27391, are not substrates Ascaris suum ?
-
?
norspermidine + O2 + H2O
-
Ascaris suum ?
-
?
norspermine + O2 + H2O
-
Ascaris suum ?
-
?
spermidine + O2 + H2O
-
Ascaris suum putrescine + 3-aminopropanal + H2O2
-
?
spermine + O2 + H2O
-
Ascaris suum spermidine + 3-aminopropanal + H2O2
-
?
tryptamine + O2 + H2O
-
Ascaris suum ?
-
?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Ascaris suum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
60 min, complete loss of activity Ascaris suum
37
-
60 min, complete loss of activity Ascaris suum
42
-
60 min, complete loss of activity Ascaris suum
56
-
60 min, complete loss of activity Ascaris suum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Ascaris suum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Ascaris suum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
MDL 27695 pH 8.5, 37°C Ascaris suum

pI Value

Organism Comment pI Value Maximum pI Value
Ascaris suum isoelectric focusing
-
7.8