Cloned (Comment) | Organism |
---|---|
subcloned and expressed in secreted and secreted-tagged forms into Escherichia coli BL21 DE3 cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MDL72527 | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
spermine | pH 8.0, 37°C, purified recombinant-tagged form enzyme | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61852 | - |
x * 61852, calculated from sequence | Mus musculus |
68000 | - |
x * 68000, secreted-tagged enzyme form, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | in mammalian cells, polyamine catabolism seems to be mediated by the activity of two enzymes, PAO and SMO | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q99K82 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in mammalian cells, polyamine catabolism seems to be mediated by the activity of two enzymes, PAO and SMO | Mus musculus | ? | - |
? | |
additional information | fails to act upon spermidine, N1-acetylpolyamines, putrescine and N1-acetylcadaverine | Mus musculus | ? | - |
? | |
spermine + O2 + H2O | - |
Mus musculus | spermidine + 3-aminopropanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61852, calculated from sequence | Mus musculus |
? | x * 68000, secreted-tagged enzyme form, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
mSMO | - |
Mus musculus |
spermine oxidase | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
spermine | pH 8.0, 37°C, purified recombinant-tagged form enzyme | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in Na-phosphate buffer | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavin/mSMO stoichiometry of 1:1 | Mus musculus |