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Literature summary for 1.5.3.13 extracted from

  • Sjogren, T.; Wassvik, C.; Snijder, A.; Aagaard, A.; Kumanomidou, T.; Barlind, L.; Kaminski, T.; Kashima, A.; Yokota, T.; Fjellstrom, O.
    The structure of murine N1-acetylspermine oxidase reveals molecular details of vertebrate polyamine catabolism (2017), Biochemistry, 56, 458-467 .
    View publication on PubMed
Search for more literature for 1.5.3.13

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of APAO in its oxidized holo form and in complex with substrate and irreversible inhibitor MDL7252 Mus musculus

Protein Variants

Protein Variants Comment Organism
N313A dramatic reduction in catalytic efficiency with substrate N1-acetylspermine Mus musculus
N313D dramatic reduction in catalytic efficiency with substrate N1-acetylspermine Mus musculus
N313L dramatic reduction in catalytic efficiency with substrate N1-acetylspermine Mus musculus
N313T dramatic reduction in catalytic efficiency with substrate N1-acetylspermine Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
MDL72527 irreversible. In addition to the covalent adduct, a second MDL72527 molecule is bound in the active site. Binding of MDL72527 is accompanied by altered conformations in the APAO backbone Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 N1-acetylspermine wild-type, pH 9, 30°C Mus musculus
0.1
-
 N1-acetylspermine mutant N313A, pH 9, 30°C Mus musculus
0.12
-
 N1-acetylspermine mutant N313T, pH 9, 30°C Mus musculus
0.2
-
 N1-acetylspermine mutant N313D, pH 9, 30°C Mus musculus
0.2
-
 N1-acetylspermine mutant N313L, pH 9, 30°C Mus musculus
6
-
 spermine mutant N313D, pH 9, 30°C Mus musculus
7
-
 spermine mutant N313L, pH 9, 30°C Mus musculus
8
-
 spermine wild-type, pH 9, 30°C Mus musculus
9
-
 spermine mutant N313A, pH 9, 30°C Mus musculus
11
-
 spermine mutant N313T, pH 9, 30°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Mus musculus 5777
-

Organism

Organism UniProt Comment Textmining
Mus musculus Q8C0L6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N1-acetylspermine + O2 + H2O
-
 Mus musculus spermidine + 3-acetamidopropanal + H2O2
-
 ?
spermine + O2 + H2O
-
 Mus musculus spermidine + 3-aminopropanal + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
APAO
-
 Mus musculus
PAOX
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2
-
 spermine mutant N313D, pH 9, 30°C Mus musculus
7.5
-
 spermine mutant N313L, pH 9, 30°C Mus musculus
8
-
 spermine wild-type, pH 9, 30°C Mus musculus
8.7
-
 spermine mutant N313T, pH 9, 30°C Mus musculus
8.8
-
 spermine mutant N313A, pH 9, 30°C Mus musculus
22
-
 N1-acetylspermine mutant N313T, pH 9, 30°C Mus musculus
25
-
 N1-acetylspermine mutant N313A, pH 9, 30°C Mus musculus
25
-
 N1-acetylspermine mutant N313L, pH 9, 30°C Mus musculus
27
-
 N1-acetylspermine mutant N313D, pH 9, 30°C Mus musculus
27
-
 N1-acetylspermine wild-type, pH 9, 30°C Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.8
-
 spermine mutant N313T, pH 9, 30°C Mus musculus
0.9
-
 spermine mutant N313D, pH 9, 30°C Mus musculus
1
-
 spermine mutant N313A, pH 9, 30°C Mus musculus
1
-
 spermine wild-type, pH 9, 30°C Mus musculus
1.1
-
 spermine mutant N313L, pH 9, 30°C Mus musculus
137
-
 N1-acetylspermine mutant N313D, pH 9, 30°C Mus musculus
144
-
 N1-acetylspermine mutant N313L, pH 9, 30°C Mus musculus
175
-
 N1-acetylspermine mutant N313T, pH 9, 30°C Mus musculus
277
-
 N1-acetylspermine mutant N313A, pH 9, 30°C Mus musculus
9600
-
 N1-acetylspermine wild-type, pH 9, 30°C Mus musculus