Application | Comment | Organism |
---|---|---|
analysis | sarcosine oxidases can be applied in enzymatic measuring of creatinine | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | strong inhibition | Bacillus sp. (in: Bacteria) | |
additional information | no inhibition by Tween 20 and Triton X-100 | Bacillus sp. (in: Bacteria) | |
SDS | strong inhibition | Bacillus sp. (in: Bacteria) | |
Tween 80 | strong inhibition | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
sarcosine | pH 8.5, 60°C | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000 | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | Bacillus sp. (in: Bacteria) | - |
glycine + formaldehyde + H2O2 | - |
? | |
sarcosine + H2O + O2 | Bacillus sp. (in: Bacteria) BSD-8 | - |
glycine + formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
isolated from soil | - |
Bacillus sp. (in: Bacteria) BSD-8 | - |
isolated from soil | - |
Purification (Comment) | Organism |
---|---|
native enzyme by ammoniumm sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | - |
Bacillus sp. (in: Bacteria) | glycine + formaldehyde + H2O2 | - |
? | |
sarcosine + H2O + O2 | - |
Bacillus sp. (in: Bacteria) BSD-8 | glycine + formaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000 | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified enzyme, stable at | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | purified enzyme, stable at | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoenzyme with noncovalently bound flavin | Bacillus sp. (in: Bacteria) |