Crystallization (Comment) | Organism |
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complexed with methylthioacetate, pyrrole 2-carboxylate and sulfite, and sarcosine-reduced enzyme. Sarcosine oxidase comprises alpha-, beta-, gamma- and delta-subunits, FAD and FMN cofactors, and a large internal cavity. Methylthioacetate and pyrrole 2-carboxylate are sandwiched between the re-face of the FAD isoalloxazine ring and the beta-subunit C-terminal residues. Reduction of flavin cofactors shifts the beta-subunit A1 residue towards the alpha-subunit M55, forming a surface cavity at the oxygen-channel vestibule and rendering the beta-subunit C-terminal residues mobile. Three channels connect the cavity and the enzyme surface. Two of them exist in the inter-subunit space between alpha- and beta-subunits, and the substrate sarcosine seems to enter the active site through either of these channels and reaches the reside of the FAD isoalloxazine ring by traversing the mobile beta-subunit C-terminal residues. The third channel goes through the alpha-subunit and has a folinic acid-binding site | Corynebacterium sp. |
Organism | UniProt | Comment | Textmining |
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Corynebacterium sp. | - |
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- |