Protein Variants | Comment | Organism |
---|---|---|
additional information | production of soluble apoenzyme lacking covalently bound FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
apoenzyme lacking FAD | Bacillus sp. (in: Bacteria) |
3 | - |
apoenzyme reconstituted with 8-nor-8-chloro-FAD | Bacillus sp. (in: Bacteria) |
27 | - |
apoenzyme reconstituted with FAD | Bacillus sp. (in: Bacteria) |
34.7 | - |
native enzyme | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | - |
Bacillus sp. (in: Bacteria) | glycine + formaldehyde + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound to wild-type. Production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD | Bacillus sp. (in: Bacteria) |