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Literature summary for 1.5.3.1 extracted from

  • Suzuki, M.
    Purification and some properties of sarcosine oxidase from Corynebacterium sp. U-96 (1981), J. Biochem., 89, 599-607.
    View publication on PubMed
Search for more literature for 1.5.3.1

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde
-
 Corynebacterium sp.
acetate
-
 Corynebacterium sp.
AgNO3 2 mM, 0% relative activity Corynebacterium sp.
Cd2+ 2 mM, 7% relative activity Corynebacterium sp.
Cu2+ 2 mM, 0% relative activity Corynebacterium sp.
Fe3+ not inhibitory Corynebacterium sp.
formaldehyde
-
 Corynebacterium sp.
Hg2+ 2 mM, 0% relative activity Corynebacterium sp.
iodoacetamide 2 mM, 4% relative activity Corynebacterium sp.
iodoacetate
-
 Corynebacterium sp.
N-ethylmaleimide 2 mM, 20% relative activity Corynebacterium sp.
p-chloromercuribenzoate 0.01 mM, 0% relative activity Corynebacterium sp.
propionate
-
 Corynebacterium sp.
sodium lauryl sulfate 2 mM, 0% relative activity Corynebacterium sp.
Zn2+ 2 mM, 7% relative activity Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 O2
-
 Corynebacterium sp.
0.31
-
 2,6-dichlorophenolindophenol
-
 Corynebacterium sp.
1.67
-
 phenazine methosulfate
-
 Corynebacterium sp.
2.7
-
 potassium ferricyanide
-
 Corynebacterium sp.
3.4
-
 sarcosine
-
 Corynebacterium sp.
8.7
-
 N-Methyl-DL-alanine
-
 Corynebacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.
21000
-
 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.
44000
-
 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.
110000
-
 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.
174000
-
 meniscus depletion method Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
 U-96
-

Purification (Commentary)

Purification (Comment) Organism
U-96 Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.93
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information very specific for oxygen as acceptor, oxygen can be replaced by 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, much smaller Vmax/Km values than for O2 Corynebacterium sp. ?
-
 ?
N-ethylglycine + O2 + H2O
-
 Corynebacterium sp. acetaldehyde + glycine + H2O2
-
 ?
N-methyl-DL-alanine + O2 + H2O
-
 Corynebacterium sp. formaldehyde + DL-alanine + H2O2
-
 ?
N-methyl-L-alanine + O2 + H2O
-
 Corynebacterium sp. formaldehyde + L-alanine + H2O2
-
 r
sarcosine + H2O + 2,6-dichlorophenolindophenol
-
 Corynebacterium sp. glycine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 r
sarcosine + H2O + phenazine methosulfate
-
 Corynebacterium sp. glycine + formaldehyde + reduced phenazine methosulfate
-
 r
sarcosine + H2O + potassium ferricyanide
-
 Corynebacterium sp. glycine + formaldehyde + potassium ferrocyanide
-
 r
sarcosine + O2 + H2O
-
 Corynebacterium sp. glycine + formaldehyde + H2O2
-
 ?

Subunits

Subunits Comment Organism
tetramer 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, complete loss of activity Corynebacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
 N-ethylglycine
-
 Corynebacterium sp.
3.4
-
 N-Methyl-L-alanine
-
 Corynebacterium sp.
5.8
-
 sarcosine
-
 Corynebacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6: about 35% of maximum activity Corynebacterium sp.
6 10 pH 10: about 70% of maximum activity Corynebacterium sp.

Cofactor

Cofactor Comment Organism Structure
flavin 1 mol of covalently bound and 1 mol of noncovalently bound flavin per mol of enzyme Corynebacterium sp.