Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.8 extracted from

  • Noda, C.; Ichihara, A.
    Purification and properties of L-lysine-alpha-ketoglutarate reductase from rat liver mitochondria (1978), Biochim. Biophys. Acta, 525, 307-313.
    View publication on PubMed
Search for more literature for 1.5.1.8

Inhibitors

Inhibitors Comment Organism Structure
L-ornithine complete inhibition of saccharopine formation, competitive with lysine Rattus norvegicus
leucine complete inhibition of saccharopine formation, competitive with lysine Rattus norvegicus
tryptophan complete inhibition of saccharopine formation Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
 NADPH
-
 Rattus norvegicus
1.4
-
 2-oxoglutarate
-
 Rattus norvegicus
2.2
-
 L-lysine
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 4 * 52000, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9, SDS-PAGE Rattus norvegicus
230000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Wistar strain
-
Rattus norvegicus male Wistar
-
 male Wistar strain
-

Purification (Commentary)

Purification (Comment) Organism
300fold purification, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.3
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
0°C, 10 mM Tris-HCl buffer, pH 7.6, 5 mM 2-mercaptoethanol, 0.1 mM EDTA, stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O lysine formation is only 3-5% of the saccharopine formation r
L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
L-lysine + 2-oxoglutarate + NADPH equilibrium favors sacharopine formation, lysine formation is only 3-5% of the saccharopine formation Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O lysine formation is only 3-5% of the saccharopine formation r
L-lysine + 2-oxoglutarate + NADPH equilibrium favors sacharopine formation, lysine formation is only 3-5% of the saccharopine formation Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O lysine formation is only 3-5% of the saccharopine formation r
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Rattus norvegicus male Wistar N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O lysine formation is only 3-5% of the saccharopine formation r
L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Rattus norvegicus male Wistar N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
additional information not as electron acceptor: NADH Rattus norvegicus ?
-
 ?
additional information lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 are separate enzymes Rattus norvegicus ?
-
 ?
additional information not as substrates: ornithine, glutamine, oxaloacetate, pyruvate Rattus norvegicus ?
-
 ?
additional information not as electron acceptor: NADH Rattus norvegicus male Wistar ?
-
 ?
additional information lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 are separate enzymes Rattus norvegicus male Wistar ?
-
 ?
additional information not as substrates: ornithine, glutamine, oxaloacetate, pyruvate Rattus norvegicus male Wistar ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 52000, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 saccharopine formation Rattus norvegicus
9
-
 L-lysine formation Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rattus norvegicus
NADPH specific for NADPH Rattus norvegicus