Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 to 15 mM: 1.7fold increase in activity, higher concentrations have no further effect, it may reduce oxidized Cys-707 or another important sulfhydryl group | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
clone expressed in insect cells has both dehydrogenase and hydrolase activity and exhibits almost identical kinetic parameters to the native rat liver enzyme | Rattus norvegicus |
FDH is cloned | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C707A | mutant with a complete loss of NADP+-dependent 10-formyltetrahydrofolate dehydrogenase activity, but no influence on hydrolase activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,10-dideazatetrahydrofolate | 0.4 mM, 98% inhibition, folate analog | Rattus norvegicus | |
additional information | not inhibited by the folate analog N-[4-[[3-(2,4-diamino-1,6-dihydro-6-oxo-5-pyrimidinyl)propyl]amino]-benzoyl]-L-glutamate | Rattus norvegicus | |
N-ethylmaleimide | 1 mM or 10 mM, complete inhibition | Rattus norvegicus | |
propanal | FDH activity decreases with increasing concentrations, competes with 10-formyltetrahydrofolate for NADP+-dependent oxidation | Rattus norvegicus | |
tetrahydrofolate | - |
Oryctolagus cuniculus | |
tetrahydrofolate | - |
Rattus norvegicus | |
Trypsin | - |
Oryctolagus cuniculus | |
Trypsin | tryptic digestion inactivates 10-formyltetrahydrofolate dehydrogenase but not hydrolase activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NADP+ | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99015 | - |
x * 99015, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | enzyme functions not as an aldehyde dehydrogenase in vivo, 10-formyltetrahydrofolate hydrolase activity is of no physiological significance, disulfiram may inhibit enzyme activity and probably perturb hepatic folate metabolism in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
prone to oxidative inactivation, but may be partially reactivated by incubation with 100 mM 2-mercaptoethanol or 100 mM dithiothreitol | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ | reaction mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-5,8-dideazafolate + NADP+ + H2O | - |
Rattus norvegicus | 5,8-dideazafolate + CO2 + NADPH + H+ | - |
? | |
10-formyl-5,8-dideazafolate + NADP+ + H2O | - |
Oryctolagus cuniculus | 5,8-dideazafolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | - |
Oryctolagus cuniculus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | mechanism which proceeds through thiohemiacetal and thioester intermediates, nucleophilic attack by the essential active site Cys-707 on the carbonyl group of 10-formyltetrahydrofolate, folate binding domain requires presence of an intact ring system | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
additional information | enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate | Rattus norvegicus | ? | - |
? | |
additional information | enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate | Oryctolagus cuniculus | ? | - |
? | |
additional information | enzyme catalyzes also NADP+-independent hydrolytic cleavage of 10-formyltetrahydrofolate to tetrahydrofolate and formate, hydrolysis is very inefficient compared to dehydrogenase reaction and may be an artifact of assay system | Rattus norvegicus | ? | - |
? | |
additional information | enzyme functions not as an aldehyde dehydrogenase in vivo, 10-formyltetrahydrofolate hydrolase activity is of no physiological significance, disulfiram may inhibit enzyme activity and probably perturb hepatic folate metabolism in vivo | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 99015, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | identical with folate binding protein cytosol-I, FBP-CI | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Oryctolagus cuniculus | |
folate | 10-formyl-5,8-dideazafolate and 5,8-dideazafolate | Rattus norvegicus | |
folate | 10-formyl-5,8-dideazafolate and 5,8-dideazafolate | Oryctolagus cuniculus | |
NADP+ | NADP+-dependent | Rattus norvegicus | |
NADP+ | NADP+-dependent | Oryctolagus cuniculus | |
NADP+ | binding of substrate, 10-formyltetrahydrofolate or propanal, influences enzyme affinity for NADP+ | Rattus norvegicus | |
tetrahydrofolate | - |
Rattus norvegicus | |
tetrahydrofolate | - |
Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
5,10-dideazatetrahydrofolate | - |
Rattus norvegicus |