Cloned (Comment) | Organism |
---|---|
the wild type and R653Q mutant pBKeDCS constructs are transformed into Escherichia coli BL21 DE3 to express the full-length protein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R653Q | mutant enzyme has normal substrate affinity but a 36% reduction in half-life at 42°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylene tetrahydrofolate + NADP+ | Homo sapiens | dehydrogenase activity of MTHFD1 | 5,10-methenyltetrahydrofolate + NADPH | - |
r | |
additional information | Homo sapiens | (MTHFD1) is a trifunctional enzyme that interconverts tetrahydrofolate derivatives for nucleotide synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P11586 | - |
- |
Purification (Comment) | Organism |
---|---|
by anion-exchange chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.2 | - |
dehydrogenase activity of the wild type enzyme | Homo sapiens |
13.9 | - |
dehydrogenase activity of the mutant R653Q | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylene tetrahydrofolate + NADP+ | dehydrogenase activity of MTHFD1 | Homo sapiens | 5,10-methenyltetrahydrofolate + NADPH | - |
r | |
additional information | (MTHFD1) is a trifunctional enzyme that interconverts tetrahydrofolate derivatives for nucleotide synthesis | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methylenetetrahydrofolate dehydrogenase | - |
Homo sapiens |
methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase | - |
Homo sapiens |
MTHFD1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |