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Literature summary for 1.5.1.5 extracted from

  • Kim, J.H.; Sung, M.W.; Lee, E.H.; Nam, K.H.; Hwang, K.Y.
    Crystallization and preliminary X-ray diffraction analysis of 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum DSM 1728 (2008), J. Microbiol. Biotechnol., 18, 283-286.
    View publication on PubMed
Search for more literature for 1.5.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermoplasma acidophilum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, in the presence of its cofactor NADP+, at 22°C using polyethylene glycol 4000 as a precipitant Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30600
-
 SDS-PAGE Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
 strain DSM 1728
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap affinity column chromatography Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+
-
 Thermoplasma acidophilum 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30600, SDS-PAGE Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase
-
 Thermoplasma acidophilum
MTHFDC
-
 Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
NAD+ prefers NADP+ as a cofactor, rather than NAD+ Thermoplasma acidophilum
NADP+ prefers NADP+ as a cofactor, rather than NAD+ Thermoplasma acidophilum