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Literature summary for 1.5.1.5 extracted from

  • Paukert, J.L.; Rabinowitz, J.C.
    Formyl-methenyl-methylenetetrahydrofolate synthetase (combined): a multifunctional protein in eukaryotic folate metabolism (1980), Methods Enzymol., 66, 616-626.
    View publication on PubMed
Search for more literature for 1.5.1.5

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Saccharomyces cerevisiae
Mg2+
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 NADP+
-
 Ovis aries
0.018
-
 NADP+
-
 Sus scrofa
0.022
-
 5,10-methylenetetrahydrofolate
-
 Sus scrofa
0.038
-
 5,10-methylenetetrahydrofolate
-
 Saccharomyces cerevisiae
0.039
-
 5,10-methylenetetrahydrofolate
-
 Ovis aries
0.11
-
 NADP+
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ and other monovalent metal cations Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 2 * 100000, SDS-PAGE Ovis aries
150000
-
 methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, gel filtration Sus scrofa
201000
-
 methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, gel filtration Saccharomyces cerevisiae
226000
-
 methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, gel filtration Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+ Sus scrofa
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
5,10-methylenetetrahydrofolate + NADP+ Saccharomyces cerevisiae
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
5,10-methylenetetrahydrofolate + NADP+ Ovis aries
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-
Saccharomyces cerevisiae
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclohydrolase and methylenetetrahydrofolate dehydrogenase combined Saccharomyces cerevisiae
trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase Sus scrofa
trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-
liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+
-
 Sus scrofa 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
5,10-methylenetetrahydrofolate + NADP+
-
 Saccharomyces cerevisiae 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
5,10-methylenetetrahydrofolate + NADP+
-
 Ovis aries 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 100000, SDS-PAGE Ovis aries

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Sus scrofa
NADP+
-
 Saccharomyces cerevisiae
NADP+
-
 Ovis aries