Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.49 extracted from

  • Watanabe, S.; Tanimoto, Y.; Yamauchi, S.; Tozawa, Y.; Sawayama, S.; Watanabe, Y.
    Identification and characterization of trans-3-hydroxy-L-proline dehydratase and DELTA(1)-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-L-proline metabolism of bacteria (2014), FEBS Open Bio, 4, 240-250.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.49

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.79
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADH Colwellia psychrerythraea
5.9
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADPH Colwellia psychrerythraea
18.3
-
 L-proline pH 10.5, 30°C, cosubstrate: NADP+ Colwellia psychrerythraea
18.8
-
 L-proline pH 10.5, 30°C, cosubstrate: NAD+ Colwellia psychrerythraea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DELTA1-pyrroline-2-carboxylate + NADH + H+ Colwellia psychrerythraea the enzyme is part of the trans-3-hydroxy-L-proline pathway L-proline + NAD+
-
 ?
DELTA1-pyrroline-2-carboxylate + NADPH + H+ Colwellia psychrerythraea the enzyme is part of the trans-3-hydroxy-L-proline pathway L-proline + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Colwellia psychrerythraea Q485S0
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00451
-
 L-proline, pH 10.5, 30°C, cosubstrate: NAD+ Colwellia psychrerythraea
0.63
-
 L-proline, pH 10.5, 30°C, cosubstrate: NADP+ Colwellia psychrerythraea
4.76
-
 DELTA1-pyrroline-2-carboxylate + NADH, pH 6.5, 30°C Colwellia psychrerythraea
30.2
-
 DELTA1-pyrroline-2-carboxylate + NADPH, pH 6.5, 30°C Colwellia psychrerythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DELTA1-pyrroline-2-carboxylate + NADH + H+ the enzyme is part of the trans-3-hydroxy-L-proline pathway Colwellia psychrerythraea L-proline + NAD+
-
 ?
DELTA1-pyrroline-2-carboxylate + NADH + H+ preference of the reaction equilibrium to the direction toward NADPH dependent reduction. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value Colwellia psychrerythraea L-proline + NAD+
-
 ?
DELTA1-pyrroline-2-carboxylate + NADPH + H+ the enzyme is part of the trans-3-hydroxy-L-proline pathway Colwellia psychrerythraea L-proline + NADP+
-
 ?
DELTA1-pyrroline-2-carboxylate + NADPH + H+ preference of the reaction equilibrium to the direction toward NADPH dependent reduction. The preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value Colwellia psychrerythraea L-proline + NADP+
-
 ?
L-proline + NAD+ preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 Colwellia psychrerythraea DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 r
L-proline + NADP+ preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 Colwellia psychrerythraea DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 r
additional information no significant activity with DELTA1-piperideine-2-carboxylate or L-pipecolate. The kcat/Km values for DELTA1-piperideine-2-carboxylate and L-pipecolate in the presence of NADP+(H) are 111fold and 223fold lower than those for DELTA1-pyrroline-2-carboxylate and L-proline, mainly caused by 90fold and 51fold lower kcat values, respectively Colwellia psychrerythraea ?
-
 ?

Synonyms

Synonyms Comment Organism
LhpH
-
 Colwellia psychrerythraea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Colwellia psychrerythraea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0069
-
 L-proline pH 10.5, 30°C, cosubstrate: NAD+ Colwellia psychrerythraea
1.01
-
 L-proline pH 10.5, 30°C, cosubstrate: NADP+ Colwellia psychrerythraea
10.35
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADH Colwellia psychrerythraea
124.5
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADPH Colwellia psychrerythraea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at, reduction of DELTA1-pyrroline-2-carboxylate Colwellia psychrerythraea
10.5
-
 assay at, oxidation of L-proline Colwellia psychrerythraea

Cofactor

Cofactor Comment Organism Structure
NAD+ in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 Colwellia psychrerythraea
NADH the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value Colwellia psychrerythraea
NADP+ in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 Colwellia psychrerythraea
NADPH the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value Colwellia psychrerythraea

General Information

General Information Comment Organism
metabolism the enzyme is part of the trans-3-hydroxy-L-proline pathway Colwellia psychrerythraea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000367
-
 L-proline pH 10.5, 30°C, cosubstrate: NAD+ Colwellia psychrerythraea
0.05519
-
 L-proline pH 10.5, 30°C, cosubstrate: NADP+ Colwellia psychrerythraea
3.7
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADH Colwellia psychrerythraea
21.1
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADPH Colwellia psychrerythraea