Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | about 14fold increase of activity at 20 mM. Without addition of dithiothreitol to the assay mixture, only 7% of the maximum activity is detected in the purified enzyme | Vibrio alginolyticus | |
additional information | EDTA (1 mM) shows no significant stimulatory effect on enzyme activity | Vibrio alginolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 24% inhibition at 5 mM | Vibrio alginolyticus | |
additional information | EDTA (1 mM) shows no significant inhibitory effect on enzyme activity; NAD+, ATP and SPD are not inhibitory at 5 mM | Vibrio alginolyticus | |
N-ethylmaleimide | 83% inhibition at 5 mM | Vibrio alginolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.51 | - |
NADPH | at pH 7.5 and 37°C | Vibrio alginolyticus | |
2.97 | - |
NADH | at pH 7.5 and 37°C | Vibrio alginolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, Zn2+, Na+ and K+ at 1 M have no effect on enzyme activity | Vibrio alginolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45100 | - |
2 * 45100, SDS-PAGE | Vibrio alginolyticus |
93500 | - |
gel filtration | Vibrio alginolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio alginolyticus | - |
- |
- |
Vibrio alginolyticus ATCC 17749 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Blue Sepharose CL-6B column chromatography, DEAE-Sepharose column chromatography, and hydroxyapatite column chromatography | Vibrio alginolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0167 | - |
crude enzyme, at pH 7.5 and 37°C | Vibrio alginolyticus |
31 | - |
after 1856fold purification, at pH 7.5 and 37°C | Vibrio alginolyticus |
Storage Stability | Organism |
---|---|
4°C, in 20 mM Tris/HCl, pH 7.5, containing 1 mM dithiothreitol and 0.02% (v/v) NaN3, 1 week, 15% loss of activity | Vibrio alginolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADH + H+ | NADH is a much poorer cofactor than NADPH | Vibrio alginolyticus | carboxynorspermidine + H2O + NAD+ | - |
? | |
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+ | - |
Vibrio alginolyticus | carboxynorspermidine + H2O + NADP+ | - |
? | |
L-aspartate 4-semialdehyde + putrescine + NADPH + H+ | 7% activity with putrescine compared to propane-1,3-diamine | Vibrio alginolyticus | carboxyspermidine + H2O + NADP+ | - |
? | |
additional information | does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde | Vibrio alginolyticus | ? | - |
? | |
additional information | does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde | Vibrio alginolyticus ATCC 17749 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45100, SDS-PAGE | Vibrio alginolyticus |
Synonyms | Comment | Organism |
---|---|---|
ASA-DAP Schiff base reductase | - |
Vibrio alginolyticus |
C-NSPD synthase | - |
Vibrio alginolyticus |
carboxynorspermidine synthase | - |
Vibrio alginolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Vibrio alginolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 78% and 59% of the maximum activity remain at 30°C and 45°C, respectively, but no activity is observed above 50°C | Vibrio alginolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.5 | - |
Vibrio alginolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 8 | 85% and 18% of the maximum activity are observed at pH 8.0 and 6.3, respectively | Vibrio alginolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
the enzyme is unstable in buffers of pH below 6.5. At pH 6.5, 50% loss of activity is observed within 12 h at 4°C | Vibrio alginolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH is a much poorer cofactor than NADPH | Vibrio alginolyticus | |
NADPH | - |
Vibrio alginolyticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vibrio alginolyticus | isoelectric focusing | 4.3 | 4.2 |
Organism | Comment | Expression |
---|---|---|
Vibrio alginolyticus | when Vibrio alginolyticus is grown in the presence of 5 mM norspermidine, the specific activity of the enzyme is reduced by about 70% | down |