Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antimycin A | 0.05 mM, 50% inhibition | Vibrio harveyi | |
additional information | no inhibition by 0.01 mM 2,4-dinitrophenol, 0.2 mM dicoumarol, 0.1 mM rotenone | Vibrio harveyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
gel filtration | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADH + H+ | Vibrio harveyi | - |
FMNH2 + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio harveyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purity was too low to permit estimation of specific activity | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | activity with FADH2 and NAD+ is 5% of the activity with FMNH2 and NAD+ | Vibrio harveyi | FADH2 + NAD+ | - |
? | |
FMN + NADH + H+ | - |
Vibrio harveyi | FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | activity with NADP+ and FMNH2 is only 10% of the activity with FMNH2 and NAD+ | Vibrio harveyi | FMNH2 + NAD+ | - |
? | |
FMN + NADPH + H+ | activity with NADP+ and FMNH2 is only 10% of the activity with FMNH2 and NAD+ | Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
additional information | activity with FAD and NADPH is 0.5% of the activity with FMNH2 and NAD+ | Vibrio harveyi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADH:FMN oxidoreductase (flavin reductase) | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Vibrio harveyi |
8.5 | - |
- |
Vibrio harveyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | bimodal, pH 5: 90% of maximal activity, pH 6.5: about 50% of maximal activity, pH 10: about 70% of maximal activity | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | activity with NADPH and FMNH2 is only 10% of the activity with FMNH2 and NAD+ | Vibrio harveyi | |
NADPH | activity with NADPH and FMNH2 is only 10% of the activity with FMNH2 and NAD+ | Vibrio harveyi |