Cloned (Comment) | Organism |
---|---|
expressed of N-terminally His-tagged HpaCSt protein in Escherichia coli | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
crystals of the refolded enzyme are obtained by adding FMN, FAD, or riboflavin to the protein solution and without the addition of flavin compound | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | - |
- |
- |
Sulfurisphaera tokodaii 7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADH + H+ | - |
Sulfurisphaera tokodaii | FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | - |
Sulfurisphaera tokodaii 7 | FMNH2 + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavin reductase HpaCSt | - |
Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | the purified refolded enzyme does not contain FMN, unlike the same enzyme expressed as a soluble protein. After the addition of FMN to the protein solution, the refolded enzyme shows a higher activity than the enzyme expressed as the soluble protein | Sulfurisphaera tokodaii |