General Stability | Organism |
---|---|
dilute solutions can be stabilized by the addition of bovine serum albumin (0.1 mg/ml) and/or by glycerol | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | - |
Escherichia coli | |
additional information | no inhibition by EDTA, desferal or dicoumarol | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
riboflavin | second substrate: NADH, pH 7.5, 23°C | Escherichia coli | |
0.0008 | - |
FAD | second substrate: NADH, pH 7.5, 23°C | Escherichia coli | |
0.0008 | - |
FMN | second substrate: NADH, pH 7.5, 23°C | Escherichia coli | |
0.0016 | - |
riboflavin | second substrate: NADPH, pH 7.5, 23°C | Escherichia coli | |
0.002 | - |
FMN | second substrate: NADPH, pH 7.5, 23°C | Escherichia coli | |
0.025 | - |
NADH | second substrate riboflavin, pH 7.5, 23°C | Escherichia coli | |
0.043 | - |
NADPH | second substrate riboflavin, pH 7.5, 23°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.36 | - |
pH 7.5, 23°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | no activity with FAD + NADPH | Escherichia coli | FADH2 + NAD+ | - |
? | |
FMN + NADH + H+ | - |
Escherichia coli | FMNH2 + NAD+ | - |
? | |
FMN + NADPH + H+ | very low activity | Escherichia coli | FMNH2 + NADP+ | - |
? | |
riboflavin + NADH + H+ | - |
Escherichia coli | reduced riboflavin + NAD+ | - |
? | |
riboflavin + NADPH + H+ | - |
Escherichia coli | reduced riboflavin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000-29000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)H:flavin oxidoreductase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
stable at pH 7.4, except at low protein concentrations | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
4.9 |