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Literature summary for 1.5.1.41 extracted from

  • Fontecave, M.; Eliasson, R.; Reichard, P.
    NAD(P)H:flavin oxidoreductase of Escherichia coli (1987), J. Biol. Chem., 262, 12325-12331.
    View publication on PubMed

General Stability

General Stability Organism
dilute solutions can be stabilized by the addition of bovine serum albumin (0.1 mg/ml) and/or by glycerol Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate
-
Escherichia coli
additional information no inhibition by EDTA, desferal or dicoumarol Escherichia coli
N-ethylmaleimide
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
riboflavin second substrate: NADH, pH 7.5, 23°C Escherichia coli
0.0008
-
FAD second substrate: NADH, pH 7.5, 23°C Escherichia coli
0.0008
-
FMN second substrate: NADH, pH 7.5, 23°C Escherichia coli
0.0016
-
riboflavin second substrate: NADPH, pH 7.5, 23°C Escherichia coli
0.002
-
FMN second substrate: NADPH, pH 7.5, 23°C Escherichia coli
0.025
-
NADH second substrate riboflavin, pH 7.5, 23°C Escherichia coli
0.043
-
NADPH second substrate riboflavin, pH 7.5, 23°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.36
-
pH 7.5, 23°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADH + H+ no activity with FAD + NADPH Escherichia coli FADH2 + NAD+
-
?
FMN + NADH + H+
-
Escherichia coli FMNH2 + NAD+
-
?
FMN + NADPH + H+ very low activity Escherichia coli FMNH2 + NADP+
-
?
riboflavin + NADH + H+
-
Escherichia coli reduced riboflavin + NAD+
-
?
riboflavin + NADPH + H+
-
Escherichia coli reduced riboflavin + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000-29000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
NAD(P)H:flavin oxidoreductase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
stable at pH 7.4, except at low protein concentrations Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
4.9