KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
reduced coenzyme F420 | pH 8.0, 30°C | Methanogenium organophilum | |
0.05 | - |
NADPH | pH 6.0, 30°C | Methanogenium organophilum | |
0.1 | - |
NADP+ | pH 8.0, 30°C | Methanogenium organophilum | |
0.1 | - |
oxidized coenzyme F420 | pH 6.0, 30°C | Methanogenium organophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Methanogenium organophilum |
90000 | - |
gradient gel electrophoresis under nondenaturating conditions | Methanogenium organophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanobacterium palustre | - |
- |
- |
Methanobacterium palustre DSM 3108 | - |
- |
- |
Methanoculleus thermophilus | - |
- |
- |
Methanoculleus thermophilus DSM 3915 | - |
- |
- |
Methanofollis liminatans | - |
- |
- |
Methanofollis liminatans DSM 4140 | - |
- |
- |
Methanogenium organophilum | P80951 | - |
- |
Methanogenium organophilum DSM 3596 | P80951 | - |
- |
Oxidation Stability | Organism |
---|---|
stable under oxic conditions | Methanogenium organophilum |
Purification (Comment) | Organism |
---|---|
- |
Methanogenium organophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
pH 8.0, 55°C, cell extract | Methanoculleus thermophilus |
0.06 | - |
pH 8.0, 37°C, cell extract | Methanofollis liminatans |
0.3 | - |
pH 8.0, 37°C, cell extract | Methanobacterium palustre |
15.1 | - |
pH 8.0, 30°C, cell extract | Methanogenium organophilum |
998 | - |
pH 8.0, 30°C, purified enzyme | Methanogenium organophilum |
Storage Stability | Organism |
---|---|
4°C or -20°C, enzyme solution is stable for several weeks | Methanogenium organophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced coenzyme F420 + NADP+ | - |
Methanoculleus thermophilus | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | - |
Methanofollis liminatans | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | - |
Methanobacterium palustre | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | the enzyme exhibits a sequential kinetic mechanism | Methanogenium organophilum | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | - |
Methanofollis liminatans DSM 4140 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | the enzyme exhibits a sequential kinetic mechanism | Methanogenium organophilum DSM 3596 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | - |
Methanobacterium palustre DSM 3108 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | - |
Methanoculleus thermophilus DSM 3915 | oxidized coenzyme F420 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Methanogenium organophilum |
Synonyms | Comment | Organism |
---|---|---|
F420-dependent NADP reductase | - |
Methanoculleus thermophilus |
F420-dependent NADP reductase | - |
Methanofollis liminatans |
F420-dependent NADP reductase | - |
Methanobacterium palustre |
F420-dependent NADP reductase | - |
Methanogenium organophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | - |
- |
Methanogenium organophilum |
37 | - |
assay at | Methanofollis liminatans |
37 | - |
assay at | Methanobacterium palustre |
55 | - |
assay at | Methanoculleus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
pH optimum for reduction of oxidized coenzyme F420 with NADPH | Methanogenium organophilum |
9.2 | - |
pH optimum for reduction of NADP+ with reduced coenzyme F420 | Methanogenium organophilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | pH 7.5: about 50% of maximal activity, pH 10.0: about 90% of maximal activity, reduction of NADP+ with reduced coenzyme F420 | Methanogenium organophilum |