Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not inhibited by iodoacetamide (20 mM), chloromercuribenzoate (20 mM) or N-ethylmaleimide (7 mM) when preincubated for 30 min in the presence of these reagents. The enzyme is not inactivated by dioxygen | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
oxidized coenzyme F420 | pH 5.5, 65°C | Archaeoglobus fulgidus | |
0.02 | - |
reduced coenzyme F420 | pH 8.0, 65°C | Archaeoglobus fulgidus | |
0.04 | - |
NADP+ | pH 8.0, 65°C | Archaeoglobus fulgidus | |
0.04 | - |
NADPH | pH 5.5, 65°C | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Archaeoglobus fulgidus |
52000 | - |
sedimentation analysis | Archaeoglobus fulgidus |
57000 | - |
gel filtration | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced coenzyme F420 + NADP+ | Archaeoglobus fulgidus | - |
oxidized coenzyme F420 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29370 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
660 | - |
pH 8.0, 80°C | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced coenzyme F420 + NADP+ | - |
Archaeoglobus fulgidus | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | the enzyme is Si face specific with respect to C5 of reduced coenzyme F420 and Si face specific with respect to C4 of NADP+. The enzyme is specific for both coenzyme F420 and NADP+/NADPH | Archaeoglobus fulgidus | oxidized coenzyme F420 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
F420-dependent NADP reductase | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
reduction of oxidized coenzyme F420 with NADPH | Archaeoglobus fulgidus |
8 | - |
reduction of NADP+ with reduced coenzyme F420 | Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | pH 4.5: about 45% of maximal activity, pH 6.5: about 35% of maximal activity, reduction of oxidized coenzyme F420 with NADPH | Archaeoglobus fulgidus |
7 | 9 | pH 7.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity, reduction of NADP+ with reduced coenzyme F420 | Archaeoglobus fulgidus |