Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | FMN at concentrations over 0.002 mM significantly inhibits the coupled reaction in both light intensity and quantum yield, and shows apparent noncompetitive and competitive inhibition patterns against NADPH and luciferase, respectively. No inhibition of the NADPH oxidation is detected under identical conditions | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the kinetic mechanism of FRP is changed to a sequential pattern with a Km(FMN) of 0.003 mM and a Km(NADPH) of 0.02 mM in a luciferase-coupled assay measuring light emission | Vibrio harveyi | |
0.008 | - |
FMN | pH 7.0, 23°C, single-enzyme spectrophotometric assay monitoring the NADPH oxidation | Vibrio harveyi | |
0.02 | - |
NADPH | pH 7.0, 23°C, single-enzyme spectrophotometric assay monitoring the NADPH oxidation | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH + H+ | Vibrio harveyi | - |
FMNH2 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
FMNH2 + NADP+ = FMN + NADPH + H+ | ping-pong kinetic pattern | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH + H+ | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FRP | - |
Vibrio harveyi |
NADPH-FMN oxidoreductase | - |
Vibrio harveyi |