Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.005 M, 14% inhibition | Vibrio harveyi | |
AMP | 0.01 M, 28% inhibition | Vibrio harveyi | |
dicoumarol | 0.000325 M, 15% inhibition | Vibrio harveyi | |
KCN | 0.01 M, 10% inhibition | Vibrio harveyi | |
N-ethylmaleimide | 0.001 M, 26% inhibition | Vibrio harveyi | |
p-hydroxymercuribenzoate | 0.001 M, 80% inhibition | Vibrio harveyi | |
rotenone | 0.0004 M, 30% inhibition | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
FMN | pH 7.0, 23°C, spectrometric assay | Vibrio harveyi | |
0.018 | - |
FMN | pH 5.6, 23°C, spectrometric assay | Vibrio harveyi | |
0.02 | - |
NADPH | pH 5.6, 23°C, spectrometric assay | Vibrio harveyi | |
0.04 | - |
NADPH | pH 7.0, 23°C | Vibrio harveyi | |
0.04 | - |
NADPH | pH 7.0, 23°C, spectrometric assay | Vibrio harveyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH + H+ | Vibrio harveyi | - |
FMNH2 + NADP+ | - |
? | |
FMN + NADPH + H+ | Vibrio harveyi No. 392 | - |
FMNH2 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Vibrio harveyi No. 392 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio harveyi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
FMNH2 + NADP+ = FMN + NADPH + H+ | the enzyme exhibits double displacement or ping-pong kinetics | Vibrio harveyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51 | - |
pH 7.0, 23°C | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADPH + H+ | - |
Vibrio harveyi | FADH2 + NADP+ | - |
? | |
FAD + NADPH + H+ | - |
Vibrio harveyi No. 392 | FADH2 + NADP+ | - |
? | |
FMN + NADPH + H+ | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
FMN + NADPH + H+ | the NADPH specific FMN reductase does not utilize NADH | Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
FMN + NADPH + H+ | - |
Vibrio harveyi No. 392 | FMNH2 + NADP+ | - |
? | |
FMN + NADPH + H+ | the NADPH specific FMN reductase does not utilize NADH | Vibrio harveyi No. 392 | FMNH2 + NADP+ | - |
? | |
additional information | the enzyme also exhibits appreciable activity with some artificial acceptors: menadione, 2,6-dichlorophenolindophenol, KFeCN6 or 5,5'-dithiobis(2-nitrobenzoic acid). Low activity with methylene blue as acceptor | Vibrio harveyi | ? | - |
? | |
additional information | the enzyme also exhibits appreciable activity with some artificial acceptors: menadione, 2,6-dichlorophenolindophenol, KFeCN6 or 5,5'-dithiobis(2-nitrobenzoic acid). Low activity with methylene blue as acceptor | Vibrio harveyi No. 392 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH specific FMN reductase | - |
Vibrio harveyi |
NADPH:FMN oxidoreductase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Vibrio harveyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
NADPH | pH 7.0, 23°C | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Vibrio harveyi |
7 | - |
assay at | Vibrio harveyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | the enzyme exhibits a maximum activity at pH 5.5 which drops to a broad shoulder from pH 6.5 to pH 8.5 with an activity 75% that of maximum at pH 7.0. About 60% of maximal activity at pH 5.0, about 50% of maximal activity at pH 10.0 | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | the NADPH specific FMN reductase does not utilize NADH | Vibrio harveyi |