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Literature summary for 1.5.1.34 extracted from

  • Wild, C.; Golderer, G.; Grobner, P.; Werner-Felmayer, G.; Werner, E.R.
    Physarum polycephalum expresses a dihydropteridine reductase with selectivity for pterin substrates with a 6-(1', 2'-dihydroxypropyl) substitution (2003), Biol. Chem., 384, 1057-1062.
    View publication on PubMed
Search for more literature for 1.5.1.34

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli Physarum polycephalum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Physarum polycephalum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 23000, about, recombinant enzyme, SDS-PAGE Physarum polycephalum
46000
-
 about, recombinant enzyme, gel filtration Physarum polycephalum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,6,7,8-tetrahydropteridine + NAD(P)+ Physarum polycephalum enzyme is required for recycling of 6,7-dihydropteridine, in vivo regulation is related to growth phase 6,7-dihydropteridine + NAD(P)H
-
 r

Organism

Organism UniProt Comment Textmining
Physarum polycephalum Q8WTJ2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration to homogeneity Physarum polycephalum

Source Tissue

Source Tissue Comment Organism Textmining
microplasmodium
-
 Physarum polycephalum
-
additional information constant enzyme expression during cell cycle of multinuclear microplasmodia, but down-regulation during development of sporangia, inhibited or reversed by glucose feeding, expression profile Physarum polycephalum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Physarum polycephalum
0.069
-
 enzyme extract of microplasmodia Physarum polycephalum
38.3
-
 purified recombinant enzyme Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6R)-tetrahydrobiopterin + NAD(P)+
-
 Physarum polycephalum ?
-
 r
(6R,S)-6-hydroxy-methyltetrahydropterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
(6R,S)-6-methyl-tetrahydropterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
(6R,S)-tetrahydrodictyopterin + NAD(P)+
-
 Physarum polycephalum ?
-
 r
(6R,S)-tetrahydrofolic acid + NAD(P)+ low activity Physarum polycephalum ?
-
 r
(6R,S)-tetrahydroneopterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
(6S)-tetrahydrobiopterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
5,6,7,8-tetrahydropteridine + NAD(P)+ enzyme is required for recycling of 6,7-dihydropteridine, in vivo regulation is related to growth phase Physarum polycephalum 6,7-dihydropteridine + NAD(P)H
-
 r
6,7-dimethyl-tetrahydropterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
additional information substrate specificity, enzyme shows selectivity for pterin substrates with a 6-(1', 2'-dihydroxypropyl) substitution Physarum polycephalum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23000, about, recombinant enzyme, SDS-PAGE Physarum polycephalum

Synonyms

Synonyms Comment Organism
DHPR
-
 Physarum polycephalum
dihydropteridine reductase
-
 Physarum polycephalum
quinoid dihydropteridine reductase
-
 Physarum polycephalum

Cofactor

Cofactor Comment Organism Structure
NAD(P)H NADH is the preferred cofactor Physarum polycephalum