Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli | Physarum polycephalum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Physarum polycephalum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, about, recombinant enzyme, SDS-PAGE | Physarum polycephalum |
46000 | - |
about, recombinant enzyme, gel filtration | Physarum polycephalum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6,7,8-tetrahydropteridine + NAD(P)+ | Physarum polycephalum | enzyme is required for recycling of 6,7-dihydropteridine, in vivo regulation is related to growth phase | 6,7-dihydropteridine + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | Q8WTJ2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration to homogeneity | Physarum polycephalum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
microplasmodium | - |
Physarum polycephalum | - |
additional information | constant enzyme expression during cell cycle of multinuclear microplasmodia, but down-regulation during development of sporangia, inhibited or reversed by glucose feeding, expression profile | Physarum polycephalum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Physarum polycephalum |
0.069 | - |
enzyme extract of microplasmodia | Physarum polycephalum |
38.3 | - |
purified recombinant enzyme | Physarum polycephalum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6R)-tetrahydrobiopterin + NAD(P)+ | - |
Physarum polycephalum | ? | - |
r | |
(6R,S)-6-hydroxy-methyltetrahydropterin + NAD(P)+ | low activity | Physarum polycephalum | ? | - |
r | |
(6R,S)-6-methyl-tetrahydropterin + NAD(P)+ | low activity | Physarum polycephalum | ? | - |
r | |
(6R,S)-tetrahydrodictyopterin + NAD(P)+ | - |
Physarum polycephalum | ? | - |
r | |
(6R,S)-tetrahydrofolic acid + NAD(P)+ | low activity | Physarum polycephalum | ? | - |
r | |
(6R,S)-tetrahydroneopterin + NAD(P)+ | low activity | Physarum polycephalum | ? | - |
r | |
(6S)-tetrahydrobiopterin + NAD(P)+ | low activity | Physarum polycephalum | ? | - |
r | |
5,6,7,8-tetrahydropteridine + NAD(P)+ | enzyme is required for recycling of 6,7-dihydropteridine, in vivo regulation is related to growth phase | Physarum polycephalum | 6,7-dihydropteridine + NAD(P)H | - |
r | |
6,7-dimethyl-tetrahydropterin + NAD(P)+ | low activity | Physarum polycephalum | ? | - |
r | |
additional information | substrate specificity, enzyme shows selectivity for pterin substrates with a 6-(1', 2'-dihydroxypropyl) substitution | Physarum polycephalum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, about, recombinant enzyme, SDS-PAGE | Physarum polycephalum |
Synonyms | Comment | Organism |
---|---|---|
DHPR | - |
Physarum polycephalum |
dihydropteridine reductase | - |
Physarum polycephalum |
quinoid dihydropteridine reductase | - |
Physarum polycephalum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | NADH is the preferred cofactor | Physarum polycephalum |