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Literature summary for 1.5.1.34 extracted from

  • Kiefer, P.M.; Varughese, K.I.; Su, Y.; Xuong, N.H.; Chang, C.F.; Gupta, P.; Bray, T.; Whiteley, J.M.
    Altered structural and mechanistic properties of mutant dihydropteridine reductases (1996), J. Biol. Chem., 271, 3437-3444.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and several mutants in Escherichia coli Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion, polyethylene glycol 4500, Tris phosphate, pH 7.8, 10% methanol, 4°C, crystals of binary complexes with NADH of recombinant wild-type, W86I, Y146F, Y146H, Y146F/K150Q and A133S mutant enzymes, 2.1-2.3 A resolution Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
A133S similar kcat for quinonoid dihydrobiopterin as wild-type Rattus norvegicus
D37I lower kcat for quinonoid dihydrobiopterin than wild-type Rattus norvegicus
K150I lower kcat for quinonoid dihydrobiopterin than wild-type Rattus norvegicus
K150M lower kcat for quinonoid dihydrobiopterin than wild-type Rattus norvegicus
K150Q lower kcat for quinonoid dihydrobiopterin than wild-type Rattus norvegicus
N186A lower kcat for quinonoid dihydrobiopterin than wild-type Rattus norvegicus
W86I lower kcat for quinonoid dihydrobiopterin than wild-type Rattus norvegicus
Y146F almost no activity with quinonoid dihydrobipterin Rattus norvegicus
Y146F/K150Q lower kcat for quinonoid dihydrobiopterin than wild-type Rattus norvegicus
Y146H lower kcat for quinonoid dihydrobiopterin than wild-type Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
quinonoid dihydrobiopterin Y146F mutant enzyme Rattus norvegicus
0.01
-
NADH Y146F mutant enzyme Rattus norvegicus
0.012
-
NADH W86I mutant enzyme Rattus norvegicus
0.013
-
NADH recombinant wild-type enzyme Rattus norvegicus
0.017
-
NADH Y146H mutant enzyme Rattus norvegicus
0.02
-
quinonoid dihydrobiopterin A133S mutant enzyme Rattus norvegicus
0.021
-
NADH K150I mutant enzyme Rattus norvegicus
0.023
-
quinonoid dihydrobiopterin Y146H mutant enzyme Rattus norvegicus
0.025
-
NADH A133S mutant enzyme Rattus norvegicus
0.026
-
quinonoid dihydrobiopterin K150I mutant enzyme Rattus norvegicus
0.027
-
quinonoid dihydrobiopterin recombinant wild-type enzyme Rattus norvegicus
0.027
-
NADH Y146F/K150Q double mutant enzyme Rattus norvegicus
0.029
-
NADH K150Q mutant enzyme Rattus norvegicus
0.03
-
quinonoid dihydrobiopterin D37I mutant enzyme Rattus norvegicus
0.033
-
quinonoid dihydrobiopterin K150Q mutant enzyme Rattus norvegicus
0.036
-
NADH D37I mutant enzyme Rattus norvegicus
0.039
-
quinonoid dihydrobiopterin K150M mutant enzyme Rattus norvegicus
0.041
-
quinonoid dihydrobiopterin Y146F/K150Q double mutant enzyme Rattus norvegicus
0.07
-
quinonoid dihydrobiopterin W86I mutant enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and several mutant enzymes Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
quinoid dihydrobiopterin + NAD(P)H
-
Rattus norvegicus tetrahydrobiopterin + NAD(P)+
-
?
quinonoid dihydrobiopterin + NADH + H+
-
Rattus norvegicus 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
quinoid dihydrobiopterin Y146F mutant enzyme Rattus norvegicus
23
-
quinoid dihydrobiopterin Y146H mutant enzyme Rattus norvegicus
26
-
quinoid dihydrobiopterin K150M mutant enzyme Rattus norvegicus
36
-
quinoid dihydrobiopterin D37I mutant enzyme Rattus norvegicus
64
-
quinoid dihydrobiopterin W86I mutant enzyme Rattus norvegicus
64
-
quinoid dihydrobiopterin K150I mutant enzyme Rattus norvegicus
76
-
quinoid dihydrobiopterin Y146F/K150Q double mutant enzyme Rattus norvegicus
90
-
quinoid dihydrobiopterin K150Q mutant enzyme Rattus norvegicus
149
-
quinoid dihydrobiopterin A133S mutant enzyme Rattus norvegicus
153
-
quinoid dihydrobiopterin recombinant wild-type enzyme Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant wild-type, D37I, W86I, Y146F, Y146H, K150Q, K150I, K150M, N186A, A133S and Y146F/K150Q mutant enzymes Rattus norvegicus