Cloned (Comment) | Organism |
---|---|
expression of wild-type, Y150H, Y150S, Y150F, Y150E, Y150K, G151S and G23D in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G151S | mutation found in children suffering from phenylketonuria due to enzyme deficiency | Homo sapiens |
G23D | mutation found in children suffering from phenylketonuria due to enzyme deficiency, inactive mutant enzyme | Homo sapiens |
Y150E | lower kcat with 6-methyl-7,8-dyhydropterin than wild-type | Homo sapiens |
Y150F | lower kcat with 6-methyl-7,8-dyhydropterin than wild-type | Homo sapiens |
Y150H | lower kcat with 6-methyl-7,8-dyhydropterin than wild-type | Homo sapiens |
Y150K | lower kcat with 6-methyl-7,8-dyhydropterin than wild-type | Homo sapiens |
Y150S | lower kcat with 6-methyl-7,8-dyhydropterin than wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
quinonoid-7,8-dihydrobiopterin | recombinant wild-type enzyme | Homo sapiens | |
0.0009 | - |
quinonoid 7,8-dihydropterin | recombinant wild-type enzyme | Homo sapiens | |
0.002 | - |
NADH | recombinant Y150S glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.002 | - |
NADH | recombinant Y150K glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.0024 | - |
NADH | recombinant Y150F mutant enzyme | Homo sapiens | |
0.004 | - |
quinonoid-7,8-dihydrobiopterin | recombinant Y150H mutant enzyme | Homo sapiens | |
0.0043 | - |
NADH | recombinant Y150H mutant enzyme | Homo sapiens | |
0.0044 | - |
NADH | recombinant Y150E glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.0049 | - |
NADH | recombinant Y150S mutant enzyme | Homo sapiens | |
0.0049 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant wild-type glutathione-S-transferase fusion enzyme | Homo sapiens | |
0.005 | - |
NADH | recombinant Y150F glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.0059 | - |
NADH | recombinant Y150H glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.0062 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant G151S mutant enzyme | Homo sapiens | |
0.0064 | - |
NADH | recombinant Y150E mutant enzyme | Homo sapiens | |
0.0075 | - |
quinonoid 7,8-dihydropterin | recombinant G151S mutant enzyme | Homo sapiens | |
0.0114 | - |
NADH | recombinant wild-type glutathione-S-transferase fusion enzyme | Homo sapiens | |
0.0172 | - |
NADH | recombinant wild-type enzyme | Homo sapiens | |
0.0181 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant wild-type enzyme | Homo sapiens | |
0.0305 | - |
quinonoid-7,8-dihydrobiopterin | recombinant Y150S mutant enzyme | Homo sapiens | |
0.0593 | - |
quinonoid-7,8-dihydrobiopterin | recombinant Y150F mutant enzyme | Homo sapiens | |
0.0819 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150H mutant enzyme | Homo sapiens | |
0.0874 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150H glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.0988 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant G23D mutant enzyme | Homo sapiens | |
0.144 | - |
quinonoid 7,8-dihydropterin | recombinant G23D mutant enzyme | Homo sapiens | |
0.149 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150E glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.165 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150F glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.2 | - |
NADH | recombinant G23D mutant enzyme | Homo sapiens | |
0.252 | - |
quinonoid-7,8-dihydrobiopterin | recombinant Y150E mutant enzyme | Homo sapiens | |
0.276 | - |
NADH | recombinant G151S mutant enzyme | Homo sapiens | |
0.283 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150S mutant enzyme | Homo sapiens | |
0.33 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150K glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.406 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150S glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
0.434 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150E mutant enzyme | Homo sapiens | |
0.48 | - |
quinonoid 6-methyl-7,8-dihydropterin | recombinant Y150F mutant enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant Y150H, Y150S, Y150F, Y150E, Y150K, G151S and G23D mutant enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dihydropteridine + NADH | - |
Homo sapiens | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + NAD+ | - |
? | |
quinoid 6-methyl-7,8-dihydropterin + NADH + H+ | - |
Homo sapiens | 6-methyl-5,6,7,8-tetrahydropterin + NAD+ | - |
r | |
quinoid dihydrobiopterin + NAD(P)H | - |
Homo sapiens | tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid 6-methyl-7,8-dihydropterin + NADH + H+ | - |
Homo sapiens | 6-methyl-5,6,7,8-tetrahydropterin + NAD+ | - |
r | |
quinonoid 7,8-dihydropterin + NADH + H+ | - |
Homo sapiens | 5,6,7,8-tetrahydropterin + NAD+ | - |
r | |
quinonoid 7,8-dihydropterin + NADPH + H+ | - |
Homo sapiens | 5,6,7,8-tetrahydropterin + NADP+ | - |
r | |
quinonoid dihydrobiopterin + NADH + H+ | - |
Homo sapiens | 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant G23D mutant enzyme | Homo sapiens | |
2.3 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150E glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
6.7 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150K glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
7.3 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150E mutant enzyme | Homo sapiens | |
7.7 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150F glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
15.3 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150F mutant enzyme | Homo sapiens | |
15.7 | - |
quinoid 7,8-dihydropterin | recombinant G23D mutant enzyme | Homo sapiens | |
16.7 | - |
quinoid 7,8-dihydrobiopterin | recombinant Y150E mutant enzyme | Homo sapiens | |
31 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant wild-type glutathione-S-transferase fusion enzyme | Homo sapiens | |
35.8 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150S mutant enzyme | Homo sapiens | |
42 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150S glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
42.2 | - |
quinoid 7,8-dihydrobiopterin | recombinant Y150H mutant enzyme | Homo sapiens | |
44.5 | - |
quinoid 7,8-dihydrobiopterin | recombinant wild-type enzyme | Homo sapiens | |
44.5 | - |
quinoid 7,8-dihydropterin | recombinant wild-type enzyme | Homo sapiens | |
49.6 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150H mutant enzyme | Homo sapiens | |
50.3 | - |
quinoid 7,8-dihydrobiopterin | recombinant Y150S mutant enzyme | Homo sapiens | |
51.2 | - |
quinoid 7,8-dihydropterin | recombinant G151S mutant enzyme | Homo sapiens | |
52.2 | - |
quinoid 7,8-dihydrobiopterin | recombinant Y150F mutant enzyme | Homo sapiens | |
60 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant Y150H glutathione-S-transferase fusion mutant enzyme | Homo sapiens | |
107 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant G151S mutant enzyme | Homo sapiens | |
227 | - |
quinoid 6-methyl-7,8-dihydropterin | recombinant wild-type enzyme | Homo sapiens |