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Literature summary for 1.5.1.34 extracted from

  • Matthews, D.A.; Varughese, K.I.; Skinner, M.; Xuong, N.H.; Hoch, J.; Trach, K.; Schneider, M.; Bray, T.; Whiteley, J.M.
    Role of aspartate-37 in determining cofactor specificity and binding in rat liver dihydropteridine reductase (1991), Arch. Biochem. Biophys., 287, 234-239.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
liver enzyme expressed in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
A6V no significant differences to wild-type Rattus norvegicus
D37I significant lower kcat Rattus norvegicus
W104F no significant differences to wild-type Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 NADH native liver enzyme Rattus norvegicus
0.017
-
 NADH A6V mutant enzyme Rattus norvegicus
0.021
-
 NADH recombinant liver enzyme Rattus norvegicus
0.027
-
 NADH W104F mutant enzyme Rattus norvegicus
0.036
-
 NADH D37I mutant enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme and recombinant wild-type, W104F, A6V and D37I mutant enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
43
-
 NADH D37I mutant enzyme Rattus norvegicus
150
-
 NADH W104F mutant enzyme Rattus norvegicus
156
-
 NADH native liver enzyme Rattus norvegicus
158
-
 NADH recombinant liver enzyme Rattus norvegicus
159
-
 NADH A6V mutant enzyme Rattus norvegicus