Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-diaminopyrimidines | overview | Leishmania major | |
dihydrobiopterin | substrate inhibition above 0.01 mM | Leishmania major | |
dihydrofolate | substrate inhibition above 0.005 mM | Leishmania major | |
dihydroneopterin | substrate inhibition | Leishmania major | |
Dihydrosepiapterin | substrate inhibition | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
folate | recombinant enzyme, pH 4.7 | Leishmania major | |
0.0019 | - |
folate | - |
Leishmania tarentolae | |
0.0019 | - |
folate | recombinant enzyme, pH 6.0 | Leishmania major | |
0.0024 | - |
folate | native enzyme, pH 6.0 | Leishmania major | |
0.0024 | - |
folate | NADPH, with folate as cosubstrate, native enzyme, pH 6.0 | Leishmania major | |
0.0026 | - |
folate | recombinant enzyme, pH 6.0 | Leishmania major | |
0.0034 | - |
dihydrofolate | recombinant enzyme, pH 6.0 | Leishmania major | |
0.0054 | - |
dihydrobiopterin | recombinant enzyme, pH 7.0 | Leishmania major | |
0.0054 | - |
dihydrobiopterin | dihydrofolate, recombinant enzyme, pH 7.0 | Leishmania major | |
0.0056 | - |
dihydrobiopterin | recombinant enzyme, pH 6.0 | Leishmania major | |
0.0061 | - |
dihydrofolate | recombinant enzyme, pH 4.7 | Leishmania major | |
0.0067 | - |
dihydrofolate | recombinant enzyme, pH 6.0 | Leishmania tarentolae | |
0.0076 | - |
dihydrobiopterin | recombinant enzyme, pH 4.7 | Leishmania major | |
0.0085 | - |
folate | recombinant enzyme, pH 7.0 | Leishmania major | |
0.0085 | - |
folate | dihydrobiopterin, recombinant enzyme, pH 4.7 | Leishmania tarentolae | |
0.00935 | - |
NADPH | with dihydrobiopterin as cosubstrate, recombinant enzyme, pH 4.7 | Leishmania tarentolae | |
0.0101 | - |
biopterin | native enzyme, pH 4.7 | Leishmania major | |
0.0109 | - |
biopterin | recombinant enzyme, pH 4.7 | Leishmania tarentolae | |
0.0116 | - |
biopterin | native enzyme, pH 4.7 | Leishmania major | |
0.012 | - |
NADPH | with dihydrofolate as cosubstrate, recombinant enzyme, pH 6.0 | Leishmania tarentolae | |
0.0122 | - |
biopterin | recombinant enzyme, pH 4.7 | Leishmania major | |
0.0122 | - |
biopterin | NADPH, with folate as cosubstrate, recombinant enzyme, pH 6.0 | Leishmania major | |
0.0123 | - |
NADPH | with biopterin as cosubstrate, recombinant enzyme, pH 4.7 | Leishmania tarentolae | |
0.0132 | - |
NADPH | with biopterin as cosubstrate, recombinant enzyme, pH 4.7 | Leishmania major | |
0.0142 | - |
NADPH | with dihydrofolate as cosubstrate, recombinant enzyme, pH 6.0 | Leishmania major | |
0.0145 | - |
NADPH | with dihydrobiopterin as cosubstrate, recombinant enzyme, pH 4.7 | Leishmania major | |
0.0146 | - |
NADPH | with folate as cosubstrate, recombinant enzyme, pH 6.0 | Leishmania tarentolae | |
0.0198 | - |
biopterin | recombinant enzyme, pH 6.0 | Leishmania major | |
0.0399 | - |
biopterin | recombinant enzyme, pH 7.0 | Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, SDS-PAGE | Leishmania tarentolae |
30000 | - |
4 * 30000, SDS-PAGE | Leishmania major |
116000 | - |
recombinant enzyme, gel filtration | Leishmania major |
117000 | - |
native enzyme, gel filtration | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leishmania tarentolae | the enzyme mediates the synthesis of tetrahydropteridines | ? | - |
? | |
additional information | Leishmania major | the enzyme mediates the synthesis of tetrahydropteridines | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Leishmania tarentolae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Leishmania major |
Storage Stability | Organism |
---|---|
-80°C, 20% glycerol, 20 mM beta-mercaptoethanol, stable | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Biopterin + NADPH | - |
Leishmania tarentolae | 5,6,7,8-Tetrahydrobiopterin + NADP+ | - |
? | |
Biopterin + NADPH | - |
Leishmania major | 5,6,7,8-Tetrahydrobiopterin + NADP+ | - |
? | |
Dihydrobiopterin + NADPH | - |
Leishmania tarentolae | ? | - |
? | |
Dihydrobiopterin + NADPH | - |
Leishmania major | ? | - |
? | |
Dihydrofolate + NADPH | - |
Leishmania tarentolae | 5,6,7,8-Tetrahydrofolate + NADP+ | - |
? | |
Dihydrofolate + NADPH | - |
Leishmania major | 5,6,7,8-Tetrahydrofolate + NADP+ | - |
? | |
Dihydroneopterin + NADPH | - |
Leishmania tarentolae | ? | - |
? | |
Dihydroneopterin + NADPH | - |
Leishmania major | ? | - |
? | |
dihydrosepiapterin + NADPH | - |
Leishmania tarentolae | ? | - |
? | |
dihydrosepiapterin + NADPH | - |
Leishmania major | ? | - |
? | |
folate + NADPH | - |
Leishmania tarentolae | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | - |
Leishmania major | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
additional information | the enzyme mediates the synthesis of tetrahydropteridines | Leishmania tarentolae | ? | - |
? | |
additional information | the enzyme mediates the synthesis of tetrahydropteridines | Leishmania major | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30000, SDS-PAGE | Leishmania tarentolae |
tetramer | 4 * 30000, SDS-PAGE | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
biopterin as substrate | Leishmania major |
4.7 | - |
dihydrobiopterin as substrate | Leishmania major |
5 | 7 | dihydrofolate as substrate | Leishmania major |
6 | - |
folate as substrate | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Leishmania tarentolae | |
NADPH | dependent on | Leishmania major |