Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.33 extracted from

  • Nare, B.; Hardy, L.W.; Beverley, S.M.
    The roles of pteridine reductase 1 and dihydrofolate reductase-thymidylate synthase in pteridine metabolism in the protozoan parasite Leishmania major (1997), J. Biol. Chem., 272, 13883-13891.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
2,4-diaminopyrimidines overview Leishmania major
dihydrobiopterin substrate inhibition above 0.01 mM Leishmania major
dihydrofolate substrate inhibition above 0.005 mM Leishmania major
dihydroneopterin substrate inhibition Leishmania major
Dihydrosepiapterin substrate inhibition Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
folate recombinant enzyme, pH 4.7 Leishmania major
0.0019
-
folate
-
Leishmania tarentolae
0.0019
-
folate recombinant enzyme, pH 6.0 Leishmania major
0.0024
-
folate native enzyme, pH 6.0 Leishmania major
0.0024
-
folate NADPH, with folate as cosubstrate, native enzyme, pH 6.0 Leishmania major
0.0026
-
folate recombinant enzyme, pH 6.0 Leishmania major
0.0034
-
dihydrofolate recombinant enzyme, pH 6.0 Leishmania major
0.0054
-
dihydrobiopterin recombinant enzyme, pH 7.0 Leishmania major
0.0054
-
dihydrobiopterin dihydrofolate, recombinant enzyme, pH 7.0 Leishmania major
0.0056
-
dihydrobiopterin recombinant enzyme, pH 6.0 Leishmania major
0.0061
-
dihydrofolate recombinant enzyme, pH 4.7 Leishmania major
0.0067
-
dihydrofolate recombinant enzyme, pH 6.0 Leishmania tarentolae
0.0076
-
dihydrobiopterin recombinant enzyme, pH 4.7 Leishmania major
0.0085
-
folate recombinant enzyme, pH 7.0 Leishmania major
0.0085
-
folate dihydrobiopterin, recombinant enzyme, pH 4.7 Leishmania tarentolae
0.00935
-
NADPH with dihydrobiopterin as cosubstrate, recombinant enzyme, pH 4.7 Leishmania tarentolae
0.0101
-
biopterin native enzyme, pH 4.7 Leishmania major
0.0109
-
biopterin recombinant enzyme, pH 4.7 Leishmania tarentolae
0.0116
-
biopterin native enzyme, pH 4.7 Leishmania major
0.012
-
NADPH with dihydrofolate as cosubstrate, recombinant enzyme, pH 6.0 Leishmania tarentolae
0.0122
-
biopterin recombinant enzyme, pH 4.7 Leishmania major
0.0122
-
biopterin NADPH, with folate as cosubstrate, recombinant enzyme, pH 6.0 Leishmania major
0.0123
-
NADPH with biopterin as cosubstrate, recombinant enzyme, pH 4.7 Leishmania tarentolae
0.0132
-
NADPH with biopterin as cosubstrate, recombinant enzyme, pH 4.7 Leishmania major
0.0142
-
NADPH with dihydrofolate as cosubstrate, recombinant enzyme, pH 6.0 Leishmania major
0.0145
-
NADPH with dihydrobiopterin as cosubstrate, recombinant enzyme, pH 4.7 Leishmania major
0.0146
-
NADPH with folate as cosubstrate, recombinant enzyme, pH 6.0 Leishmania tarentolae
0.0198
-
biopterin recombinant enzyme, pH 6.0 Leishmania major
0.0399
-
biopterin recombinant enzyme, pH 7.0 Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
4 * 30000, SDS-PAGE Leishmania tarentolae
30000
-
4 * 30000, SDS-PAGE Leishmania major
116000
-
recombinant enzyme, gel filtration Leishmania major
117000
-
native enzyme, gel filtration Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leishmania tarentolae the enzyme mediates the synthesis of tetrahydropteridines ?
-
?
additional information Leishmania major the enzyme mediates the synthesis of tetrahydropteridines ?
-
?

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-
Leishmania tarentolae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant enzyme Leishmania major

Storage Stability

Storage Stability Organism
-80°C, 20% glycerol, 20 mM beta-mercaptoethanol, stable Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Biopterin + NADPH
-
Leishmania tarentolae 5,6,7,8-Tetrahydrobiopterin + NADP+
-
?
Biopterin + NADPH
-
Leishmania major 5,6,7,8-Tetrahydrobiopterin + NADP+
-
?
Dihydrobiopterin + NADPH
-
Leishmania tarentolae ?
-
?
Dihydrobiopterin + NADPH
-
Leishmania major ?
-
?
Dihydrofolate + NADPH
-
Leishmania tarentolae 5,6,7,8-Tetrahydrofolate + NADP+
-
?
Dihydrofolate + NADPH
-
Leishmania major 5,6,7,8-Tetrahydrofolate + NADP+
-
?
Dihydroneopterin + NADPH
-
Leishmania tarentolae ?
-
?
Dihydroneopterin + NADPH
-
Leishmania major ?
-
?
dihydrosepiapterin + NADPH
-
Leishmania tarentolae ?
-
?
dihydrosepiapterin + NADPH
-
Leishmania major ?
-
?
folate + NADPH
-
Leishmania tarentolae 5,6,7,8-tetrahydrofolate + NADP+
-
?
folate + NADPH
-
Leishmania major 5,6,7,8-tetrahydrofolate + NADP+
-
?
additional information the enzyme mediates the synthesis of tetrahydropteridines Leishmania tarentolae ?
-
?
additional information the enzyme mediates the synthesis of tetrahydropteridines Leishmania major ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 30000, SDS-PAGE Leishmania tarentolae
tetramer 4 * 30000, SDS-PAGE Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
biopterin as substrate Leishmania major
4.7
-
dihydrobiopterin as substrate Leishmania major
5 7 dihydrofolate as substrate Leishmania major
6
-
folate as substrate Leishmania major

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Leishmania tarentolae
NADPH dependent on Leishmania major